Free radicals are molecules with one or more unpaired electron(s) commonly found in seminal plasma. Physiologically, free radicals control sperm maturation, capacitation and hyperactivation, the acrosome reaction, and sperm-oocyte fusion. Pathologically, free radicals induce lipid peroxidation, DNA damage and apoptosis of spermatozoa. The present review deals with both the beneficial and detrim...

Hyaluronidase, acrosin and N-acetylhexosaminidase activities were examined in sperm collected from 12 beagle dogs and in culture medium after 0.5 hr and 7 hr of sperm incubation. The activities of the three enzymes were significantly higher at 7 hr than at 0.5 hr (P < 0.05, 0.01), and the increases were associated with sperm capacitation. It was considered that the three enzymes in the dog sper...

The relationship between anionic-lipid concentration and the functional properties of plasma-membrane domains was explored using the guinea-pig sperm membrane as a model, with polymyxin B (PXB) as a probe. Areas of plasmalemma specialized for fusion during the acrosome reaction had a higher affinity for the probe than adjacent nonfusigenic regions. In addition, capacitation--a process preceding...

Cryopreservation is partially damaging and induces capacitation-like changes in spermatozoa. Seminal plasma (SP) contains a variety of biochemical components, such as protein and lipids, which are specific for the regulation of sperm cell function including those effective for decapacitation of spermatozoa. Therefore, this study tested the hypothesis that desalted and lyophilized SP could preve...

Quassia amara is a medicinal plant with several pharmacological properties. There are preliminary reports implicating this plant in male reproductive toxicology. Single daily oral administration of methanol extract of Quassia amara stem bark for 6 weeks resulted in a significant reduction in the weight of the testes, caudal epididymis and seminal vescicle. Caudal epididymal sperm parameters sho...

To obtain a high in vitro fertilization rate while ensuring the ideal development of the&#x0D; embryos, thus eliminating most critical problems assisted reproductive&#x0D; technology (ART) (implantation and low pregnancy rates). Therefore, sperm taken&#x0D; from caudal epididymis must undergo acquisition progressive motility and&#x0D; fertilization. This process takes place through series physi...

Housing ensures that everyone in society has a place to live. This study explored the engagement of Local Government Units (LGUs) Capiz turnover Yolanda Permanent Projects (YPHP) toward safe and resilient shelter under Participatory Capacity Development framework. A descriptive-qualitative approach leaning on intrinsic case design was utilized. Data collection through Key Informant Interviews, ...

This study aimed to determine the most reliable method to evaluate capacitation of buffalo frozen/thawed sperm. Frozen/thawed sperm were incubated in TALP medium in absence of capacitating agents (control) and in presence of 10 μg/ml heparin for 2 and 4 hrs. Capacitation was assessed by Trypan blue/Giemsa after lysophosphatidylecholine (LPC) exposure, chlortetracycline (CTC) fluorescence assay ...

The aim of this study was to determine whether flow cytometric evaluation of combined merocyanine 540 and Yo-Pro 1 (M540-YP) staining would identify viable dog sperm that had undergone membrane stabilization known to be associated with capacitation in other species, and whether such destabilization is detected earlier than when using the tyrosine phosphorylation and ethidium homodimer (TP-EH) s...