In the past 10 years evidence - based medicine (EBM) has developed well in all aspects of allopathic medicine giving a new dimension to patient care including cost reduction and attention to medical ethics. With the public and medical community return to complementary and alternative medicine (CAM) in the last two decades there has been a call for evidence - based CAM … in the same way that all...

In the past 10 years evidence - based medicine (EBM) has developed well in all aspects of allopathic medicine giving a new dimension to patient care including cost reduction and attention to medical ethics. With the public and medical community return to complementary and alternative medicine (CAM) in the last two decades there has been a call for evidence - based CAM … in the same way that all...

A number of point mutations in the intracellular Ca2+-sensing protein calmodulin (CaM) are arrhythmogenic, yet their underlying mechanisms are not clear. These mutations generally decrease Ca2+ binding to CaM and impair inhibition of CaM-regulated Ca2+ channels like the cardiac Ca2+ release channel (ryanodine receptor, RyR2), and it appears that attenuated CaM Ca2+ binding correlates with impai...

background and aims. cad/cam systems facilitate the use of zirconia substructure materials for all-ceramic copings. this in vitro study investigated the marginal and internal fit of zirconia copings made with cad/cam system and slip-casting method. materials and methods. sixteen cad/cam made zirconia copings and 16 slip-cast made zirconia copings were fabricated and cemented with glass-ionomer ...

The angiogenesis activity was involved in many physiological and pathological conditions. The research on angiogenesis activity was increased and many new methods are come for the evaluation of the angiogenesis activity. Previously Robert Auerbach et al., described the methods used for the evaluation of angiogenesis activity. We added some more techniques to that which are currently used for th...

BACKGROUND Calmodulin (CaM) mutations have been identified recently in subjects with congenital long QT syndrome (LQTS) or catecholaminergic polymorphic ventricular tachycardia (CPVT), but the mechanisms responsible for these divergent arrhythmia-susceptibility syndromes in this context are unknown. We tested the hypothesis that LQTS-associated CaM mutants disrupt Ca2+ homeostasis in developing...

Autophosphorylation of alpha-Ca(2+)/calmodulin-dependent protein kinase II (CaM kinase II) at Thr(286) results in calmodulin (CaM) trapping, a >10,000-fold decrease in the dissociation rate of CaM from the enzyme. Here we present the first site-directed mutagenesis study on the dissociation of the high affinity complex between CaM and full-length CaM kinase II. We measured dissociation kinetics...

AIMS The present study investigated the detailed mechanism by which fractalkine (Fkn), a CX3C chemokine, induces angiogenesis and its functional implication in alleviating ischaemia in vivo. METHODS AND RESULTS Fkn induced new vessel formation on the excised rat aorta and chick chorioallantoic membrane (CAM) through CX3CR1 activation. Immunoblotting analysis, promoter assay and electrophoreti...

5'-O-Tritylinosine (KIN59) is an allosteric inhibitor of the angiogenic enzyme thymidine phosphorylase. Previous observations showed the capacity of KIN59 to abrogate thymidine phosphorylase-induced as well as developmental angiogenesis in the chicken chorioallantoic membrane (CAM) assay. Here, we show that KIN59 also inhibits the angiogenic response triggered by fibroblast growth factor-2 (FGF...