We have isolated cosmid clones containing the chromosomal chicken progesterone receptor gene. The gene consists of eight exons and is approximately 38 kilobases long. Individual exons correlate well with conserved functional domains of the receptor molecule. Alternative polyadenylylation in the second intron results in a putative non-hormone-binding protein. The cap site of the gene is heteroge...

Lucian Farcal 1,9, Francois Busquet 2, Sandra Coecke 3, Ioana Hristescu 4, Christophe Chesné 5, Christian Pellevoisin 6, Adriana Orasanu 7, Zorita Diaconeasa 8, Adrian Oros 8, Adela Pintea 8 and Carmen Socaciu 8 BIOTOX Srl, Cluj-Napoca, Romania; Center for Alternatives to Animal Testing, CAAT-Europe, University of Konstanz, Germany; DG JRC-Institute for Health and Consumer Protection, European ...

Description 43 exons spanning 80 kb with 4365 bp open reading frame (Lo Ten Foe et al., 1996). The 5-prime region upstream of the putative transcription start site of FANCA has a GC-rich region instead of TATA or CAAT boxes, which is typical of housekeeping genes (Ianzano et al., 1997). Numerous Alu repeats are present in the FANCA gene, suggesting that Alu-mediated recombination may be an impo...

One of the major cipher symmetric algorithms is AES. Its main feature is to use S-BOX step, which is the only non-linear part of this standard possessing fixed structure. During the previous studies, it was shown that AES standard security was increased by changing the design concepts of S-BOX and production of dynamic S-BOX. In this paper, a change of AES standard security is studied by produc...

The human lysosomal cysteine proteinases, cathepsins H, L, and B, have been mapped to chromosomes 15, 9, and 8, respectively, and the genomic structures of cathepsins L and B have been determined. We report here the chromosomal localization and partial gene structure for a recently sequenced human cysteine proteinase, cathepsin S. A 20-kilobase pair genomic clone of the human cathepsin S gene w...

Mer belongs to the Tyro 3 family of receptor tyrosine kinases (RTKs). Together with Axl and Rse, the three RTKs are believed to play important functional roles in the male gonads because gene knockout male mice lacking all of these receptors are infertile. In the present study, postnatal expression of Axl and Rse in mouse testes decreased during maturation while expression of Mer increased age-...

Bamacan is a chondroitin sulfate proteoglycan that abounds in basement membranes. To gain insights into the bamacan gene regulation and transcriptional control, we examined the genomic organization and identified the promoter region of the mouse bamacan gene. Secondary structure analysis of the protein reveals a sequential organization of three globular regions interconnected by two alpha-helix...

A distal transcriptional enhancer has been previously reported upstream of the mouse renin gene. A homologous sequence is also present upstream of the human renin gene, but the mouse and human renin enhancers differ markedly in their ability to activate transcription of a renin promoter. Although the 2 enhancers share high homology in their 202-bp promoter distal portions, their 40-bp proximal ...

Nkx3.1 is a prostate-specific homeobox gene related strongly to prostate development and prostate cancer. To study its regulation of transcription, 1.06 kb 5' flanking region of Nkx3.1 gene and its 5' deletion mutants (861, 617, 417 and 238 bp) were obtained by PCR and cloned into pGL(3)-basic, a promoter-less luciferase reporter vector, to examine their promoter activities driving the reporter...