In this study, a dose-response assessment was performed to understand the relation between supplementation of media with L-ascorbic acid or vitamin C and porcine oocyte maturation and the in vitro development of parthenotes (PA) and handmade cloned (HMC) embryos. Various concentrations (0, 25, 50 and 100 μg/ml) of vitamin C supplemented in in vitro maturation (IVM) and culture (IVC) media were ...

the rate of listeria abortion in tehran was investigated. abortion samples (200) were cold enriched at 4°c and subcultured on selective culture media containing acriflavin, nalidixic acid and potassium thiocyanate. sera of patients were tested serologically (if method) for screening, and results were confirmed by culturing the positive samples. antibody against l. mollocytogelles was obtained i...

Clostridium difficile is a recognized cause of pseudomembranous (antimicrobial agent-associated) colitis and may be one of the causes of antimicrobial agent-induced diarrhea. A selective and differential agar medium that contains cycloserine, cefoxitin, fructose, and egg yolk (CCFA) was developed to facilitate the isolation of C. difficile from fecal specimens. Quantitative cultures of 16 stock...

Growth of Cryptococcus neoformans from the sputum of patients with acquired immunodeficiency syndrome may be obscured by oral contamination with Candida albicans on conventional media. We prospectively compared direct plating of sputum and urine onto birdseed agar and compared birdseed agar plating with plating onto Mycosel and Sabouraud dextrose agar cultures. Thirty-two sputum and three urine...

The production of chlamydospores is a diagnostic tool used to identify Candida albicans; these structures also represent a model for morphogenetic research. The time required to produce them with standard methods is 48-72 hours in rice meal agar and tensoactive agents. This time can be shorted using liquid media such as cornmeal broth (CMB) and dairy supplements. Five media were tested: CMB plu...

Schaedler agar (SA) and Trypticase soy-yeast extract agar (TSYEA), both supplemented with rabbit blood (5%, v/v) and menadione (0.5 mg/liter), were compared with respect to quantitative recovery, quality of growth, and rapidity of growth of selected anaerobic bacteria. The media were stored for 2 to 4 days prior to use in an anaerobic glove box, where all subsequent bacteriological procedures w...

AIM Culture remains important for the detection and typing of Clostridium difficile. Culture of C. difficile spores can be enhanced on media supplemented with a germinant. Despite this, unsupplemented media continues to be used in some laboratories. The aim of this study was to quantify the effect of the known germinant sodium taurocholate on recovery of C. difficile spores and to determine if ...

Sixteen isolates of C. musae were collected from different banana growing areas of Tamil Nadu and their pathogenicity was proved under laboratory conditions. Effect of different pH levels, temperature, light intensity and media were tested against the growth of C. musae under in vitro. Results indicated that the growth of C. musae was maximum at pH range of 6.50-7.00 and temperature range of 25...

18 Objectives: To monitor the stability of rifampicin (RIF) in Löwenstein-Jensen medium (L-J 19 medium) and 7H9 broth, which are the commonly used media for drug susceptibility testing (DST) 20 for Mycobacterium tuberculosis. Methods: Rifampicin degradation in stock solution, 7H9 broth, 21 L-J medium, and during the inspissation process for L-J medium preparation was serially 22 monitored by hi...