Pyrrolobenzodiazepines (PBDs) are covalent-binding DNA-interactive agents with growing importance as payloads in Antibody Drug Conjugates (ADCs). Until now, PBDs were thought to covalently bond to C2-NH2 groups of guanines in the DNA-minor groove across a three-base-pair recognition sequence. Using HPLC/MS methodology with designed hairpin and duplex oligonucleotides, we have now demonstrated t...

Defensins are small antimicrobial peptides (AMPs) that play an important role in the innate immune system of mammals. Since the effect of mycotoxin contamination of food and feed on the secretion of intestinal AMPs is poorly understood, the aim of this study was to elucidate the individual and combined effects of four common Fusarium toxins, deoxynivalenol (DON), nivalenol (NIV), zearalenone (Z...

Polo-like kinase 1 (Plk1) plays several roles in cell division and it is a recognized cancer drug target. Plk1 levels are elevated in cancer and several types of cancer cells are hypersensitive to Plk1 inhibition. Small molecule inhibitors of the kinase domain (KD) of Plk1 have been developed. Their selectivity is limited, which likely contributes to their toxicity. Polo-like kinases are charac...

OBJECTIVE To generate recombinant Bacillus subtilis (B. subtilis) engineered for expression of porcine β-defensin-2 (pBD-2) and cecropin P1 (CP1) fusion antimicrobial peptide and investigate their anti-bacterial activity in vitro and their growth-promoting and disease resisting activity in vivo. METHODS The pBD-2 and CP1 fused gene was synthesized using the main codons of B. subtilis and inse...

This file is a facsimile of an obituary for Erik Johan Burman, published in the Acta (Proceedings) Uppsala-based Societas Regia Literaria et Scientiarum (Royal Society Learning and Sciences, or Kungl. Vetenskaps-Societeten i Uppsala) year 1730.

Programming-by-demonstration (PBD) is a new paradigm for programming industrial robots enabled by the development of the DLR/KUKA light-weight robot. Although the PBD approach facilitates and simplifies the generation of robot programs, the technician still needs to have skills and knowledge about the robotic system in order to produce efficient trajectories and to exploit the abilities of the ...

Response surface methodology was applied to optimize medium constituents for enzymatic production of L-cysteine from DL-2-amino-D-thiazoline-4-carboxylic acid (DL-ATC) by a novel Pseudomonas sp. Zjwp-14. With the Plackett-Burman design experiment, glycerol, DL-ATC, yeast extract, and pH were found to be the most powerful factors among the eight tested variables that influence intracellular enzy...

Marine actinomycetes were isolated from sediment samples collected from Pitchavaram mangrove ecosystem situated along the southeast coast of India. Maximum actinomycete population was noted in rhizosphere region. About 38% of the isolates produced L-asparaginase. One potential strain KUA106 produced higher level of enzyme using tryptone glucose yeast extract medium. Based on the studied phenoty...

Let p and 2p − 1 be prime powers and p ≡ 3 (mod 4). We describe a construction of a series of Siamese twin designs with Menon parameters (4p, 2p − p, p − p) intersecting in a derived design with parameters (2p − p, p − p, p − p − 1), and a pairwise balanced design PBD(2p − p, {p, p − p}, p − p − 1). When p and 2p − 1 are primes, the derived design and the pairwise balanced design are cyclic. Fu...

Pentaksiran Bilik Darjah (PBD) merupakan sebuah pentaksiran secara berterusan dalam pembelajaran dan pemudahcaraan (PdPc) bagi mengesan perkembangan, mengenal pasti kekuatan kelemahan serta meningkatkan pencapaian murid pembelajaran. Justeru, kajian ini mengkaji tentang tahap kefahaman, kemahiran sikap guru bahasa Melayu sekolah rendah pelaksanaan PBD. Kajian menggunakan pendekatan kuantitatif ...