There is a serious problem with the reduction of male reproductive performance of the livestock in the world. We have a hypothesis that the splicing error-caused derivation of aberrant sperm motility-related proteins may be one of its causal factors. It is thought that fresh testicular tissues are necessary for the detection of splicing errors of the mRNA. However, it is difficult to obtain tes...

Seminal plasma (SP) plays an important role in the motility, viability and maintenance of the fertilizing capacity of mammalian spermatozoa. This study is the first on brown bear (Ursus arctos) SP components, and has two main objectives: 1) to define the SP composition in bear ejaculate and 2) to identify variations in SP composition in relation to high and low levels of testosterone in serum d...

In the current study, we have developed an easy, practical, and reliable method to collect semen from camel bulls. Simply, intromission ofa super-sensitive artificial insemination glove intravaginally with outer fixation in perineal region lateral dorsal sides of thevulva, mating, withdrawal collection were successive steps. Bull behaviour before during mating process wasobserved. The collected...

FEMALE GUINEA PIGS WERE INJECTED WITH THE FOLLOWING MATERIALS: homogenates of guinea pig testis in saline or in adjuvant; suspensions of washed guinea pig sperm in saline or in adjuvant; homogenates of rabbit testis in adjuvant; guinea pig sperm and rabbit sperm in adjuvant. Control animals were not injected or were injected with adjuvant alone. At various times between 15 and 39 days after inj...

The acrosome reaction in vertebrate sperm has been shown to be essential for fertilisation [ 11. The process involves fusion and vesiculation of sperm head plasma membranes and the underlying outer acrosomal membrane, resulting in the exocytotic release of acrosomal enzymes. Yanagamachi and Usui have demonstrated that sperm have an absolute requirement for Ca*’ during the acrosome reaction [2]....

This study was designed to compare the effect of straw size (0.25 vs. 0.5 ml) and thawing time (30 vs. 60 sec) on the quality of cryopreserved buffalo bull semen. Sperm motility, plasma membrane integrity and viability were higher (p ≤ 0.05) in 0.25 ml than 0.5 ml straw, thawed at 37°C either for 30 or 60 sec. In conclusion, cryopreservation of buffalo semen in 0.25 ml straw resulted in a highe...

25 Introduction 25 Materials and methods 27 Results 32 Discussion 35 Chapter 4: Procedure for bovine ICSI, not sperm freeze-drying, impairs the function of the microtubule-organizing center 39 Abstract 39 Introduction 39 Materials and methods 40 Results 43 Discussion 47 Chapter 5: Adverse effect of cake collapse on the functional integrity of freeze-dried bull spermatozoa 49 Abstract 49 Introdu...

Indonesia has two National Artificial Insemination Centers (AIC) and more than 15 Regional (RAIC) spread across several provinces. Bulls in the AIC must have a high libido produce good quality semen. This study examines correlation between with semen testosterone concentration to determine potential frozen production from Bali bulls South Sulawesi RAIC. Ten bull were used this study. Semen coll...