Background: Artificial insemination (AI) has been used for increasing genetic potential of animals in production while application of AI has been restricted in buffalo due to low freezing ability and fertility of frozen-thawed buffalo spermatozoa. So improvement in buffalo sperm cryopreservation may enhance the breeding programs of this valuable farm animal. Semen processing is one of the major...

to study the seasonal variation in the characteristics of the azarbaijani buffalo semen, three 2-4-year-oldbulls of azarbaijani water buffaloes, kept in the buffalo breeding center, in urmia, northwest of iran, wereselected. semen samples were collected once a week for a period of one year using artificial vagina and abuffalo cow as a dummy. semen volume, colour, ph and motility and spermatozoa...

Faecal samples were collected from seventy-eight diarrhoeic cow and buffalo calves between November 1998 and February 1999 to study the genomic diversity and prevalence of Rotavirus infection by ribonucleic acid polyacrylamide gel electrophoresis (RNA-PAGE) and enzyme-linked immunosorbent assay (ELISA). In the organised dairy farm (where daily production and health records were maintained), the...

The ubiquity and importance of parasite co-infections in populations of free-living animals is beginning to be recognized, but few studies have demonstrated differential fitness effects of single infection versus co-infection in free-living populations. We investigated interactions between the emerging bacterial disease bovine tuberculosis (BTB) and the previously existing viral disease Rift Va...

Parathyroid hormone-related protein (PTHrP) is involved in the deposition of milk calcium in mammal lactation, but its role in buffalo is unclear. In this study, the full-length coding sequence of the water buffalo PTHrP gene was first isolated using reverse transcription-polymerase chain reaction. The protein was then subjected to molecular characterization using bioinformatic methods, and the...

this study was conducted to determine chromatin integrity and dna damage by dna electrophoresis and comet assays of buffalo fresh and frozen semen. semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and dna damage. a significant variation was found in semen parameters after thawing. highly sig...

Abstract As part of the agricultural sector, people in Sijunjung district also make livestock subsector family’s economic source, where type that has been cultivated for a long time is buffalo. one areas which potential to develop buffalo farms because it highest population. This study aims determine model raising, and income farmers District analyze factors affect farming District. research me...

The businesses community of different sectors affected by current energy crisis in Pakistan tends towards dairy business. They are highly interested by the information on milk composition particularly of buffalo milk due to its major contribution in national’s milk production i.e. 63% according to the FAO’s published data of 2010. It is necessary to know for maximum value addition in dairy food...

Bovine spongiform encephalopathy, a member of transmissible spongiform encephalopathies, has not been reported in buffaloes, Bubalus bubalis. Prion protein (PrP), encoded by the prion protein gene (PRNP), is fundamental in the pathogenesis of transmissible spongiform encephalopathies. We previously showed that buffaloes express more PrP proteins but lower PRNP mRNA than cattle in several pivota...

fatty liver is a metabolic disorder of dairy cows in early lactation, and approximately half of multi-parous dairy cows experience a moderate to severe fatty liver at calving. since the occurrence of fatty liver in buffalo is not known, the aim of this study was to evaluate the hepatic content of triacylglycerols (tag) and total lipids (tl), and the serum content of non-esterified fatty acids (...