Exit from mitosis must not occur prior to partitioning of chromosomes between daughter cells. We find that the GTP binding protein Tem1, a regulator of mitotic exit, is present on the spindle pole body that migrates into the bud during S phase and mitosis. Tem1's exchange factor, Lte1, localizes to the bud. Thus, Tem1 and Lte1 are present in the same cellular compartment (the bud) only after th...

We searched for bioactive substances involved in flower bud formation of Arabidopsis thaliana. some significantly decreasing HPLC peaks were detected in the extract of flower buds-forming A. thaliana compared with that of the non-flower bud-forming stage. Compound 1, which corresponded to the most decreased HPLC peak, was isolated from aerial parts of A. thaliana. From NmR and ms data, compound...

Polarized cells frequently use diffusion barriers to separate plasma membrane domains. It is unknown whether diffusion barriers also compartmentalize intracellular organelles. We used photobleaching techniques to characterize protein diffusion in the yeast endoplasmic reticulum (ER). Although a soluble protein diffused rapidly throughout the ER lumen, diffusion of ER membrane proteins was restr...

The associations between inhaled corticosteroid (ICS) use and pulmonary infections remains controversial. Chlamydia pneumoniae (C. pneumoniae) accounts for asthma exacerbations; however, there are no data regarding ICS effects on C. infections. Thus, we investigated whether fluticasone propionate (FP) or budesonide (BUD) could affect infection in vitro vivo, focusing the possible mechanisms tha...

considering the importance of grafted seedlings for the development of pecan orchards in subtropical regions of iran, and in order to achieve a scientifically practical method of producing grafted pecan seedlings, this project was carried out in safiabad agricultural research center of dezful during 2011-12. the project investigated three grafting methods including hot cable, hypocotyl and late...

new study identifies a possible new function for endostatin, the potential cancer-killer that checks blood vessel growth. Within the developing kidney, endostatin may help regulate the branching that establishes the network of urine-collecting tubules. Cells in the ureteric bud divide and change shape to form the plumbing system of the kidney. Because endostatin inhibits blood vessel formation,...

In Saccharomyces cerevisiae, Golgi elements are present in the bud very early in the cell cycle. We have analyzed this Golgi inheritance process using fluorescence microscopy and genetics. In rapidly growing cells, late Golgi elements show an actin-dependent concentration at sites of polarized growth. Late Golgi elements are apparently transported into the bud along actin cables and are also re...

In humans and mice, mutations in the Ret gene result in Hirschsprung's disease and renal defects. In the embryonic kidney, binding of Ret to its ligand, Gdnf, induces a program of epithelial cell remodeling that controls primary branch formation and branching morphogenesis within the kidney. Our previous studies showed that transcription factors belonging to the retinoic acid (RA) receptor fami...