Members of the genus Brucella are categorized as biothreat agents and pose a hazard for both humans and animals. Current identification methods rely on biochemical tests that may require up to 7 days for results. We sequenced the 16S rRNA genes of 65 Brucella strains along with 17 related strains likely to present a differential diagnostic challenge. All Brucella 16S rRNA gene sequences were de...

Smooth lipopolysaccharide (S-LPS) and lipid A of Brucella abortus and Brucella melitensis induced the production of nitric oxide (NO) by rat adherent peritoneal cells, but they induced lower levels of production of NO than Escherichia coli LPS. The participation of the inducible isoform of NO synthase (iNOS) was confirmed by the finding of an increased expression of both iNOS mRNA and iNOS prot...

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

Although Brucella endocarditis is a rare complication of human brucellosis, it is the main cause of the mortality in this disease. Traditionally, the therapeutic approach to endocarditis caused by Brucella species requires a combination of antimicrobial therapy and valve replacement surgery. In the literature, only a few cases of mitral prosthetic valve endocarditis caused by Brucella species h...

During investigations to ascertain the mechanism involved in the differential bacteriostatic effect of dyes on the growth of the three species of Brucella, it became apparent that relevant data could be obtained by studying the comparative metabolism of glutamic acid by the three species. This was initiated using resting cell techniques and determining the metabolites by paper chromatography. T...

that go to make the differ eSj such a and another, as very feVyT ( . digital m genital cataract, alkaptonu i'ur of the irl ' Nation, and probably the c.. lines. stated to run upon Mendelian .g dlfficUlt The subject of human her^ ̂ is, &t a and hence investigation R?tly anothe ?aiV8 speed. Only very character has been added t n and H11. blood group factor. Von Dung'er ̂ agglutinins ^ld have conclu...

or 1Hand what are their individual significance and value ? 4. Do the titres of ' 0 ' and ' H ' agglutinins vary or fluctuate in subsequent weeks ? If so, do they undergo similar fluctuation or independent of one another ? If such variations occur, what is their clinical significance ? 5. Are these variations, fluctuations, etc., of any value in forecasting the course of the disease, and progno...

coccus suspension with immune serum at 1,800 r.p.m. for 10 minutes, and obtained very encouraging results. Mayer and Dowling (1945) used a centrifugal method for the detection of agglutinins against meningococci and obtained highly specific results. Feldman (1946) used a similar method in the identification of cultures, specially those of the dysentery group. The present paper gives the results...

In forensic and Medical communities, empirical evidences (in any form) collected from scene of occurrence continues to validate the premises of persistence and identical. Salivary fluid is an exocrine stain encountered as evidences from scene of crime, whose analysis may link the accused and eliminate the innocents. In the present study, 130 samples of Jaats from District Meerut and Muzafarnaga...