Myotonic dystrophy (DM1) is the most common form of adult muscular dystrophy and is inherited as an autosomal dominant trait. The genetic basis of DM1 is the expansion of a CTG repeat in the 3' untranslated region of a protein kinase gene (DMPK). The molecular mechanism by which this expanded repeat produces the pathophysiology of DM1 remains unknown. Transcripts from the expanded allele accumu...

Cinnamomum camphora, a member of the Lauraceae family, is a valuable aromatic and timber tree that is indigenous to the south of China and Japan. All parts of Cinnamomum camphora have secretory cells containing different volatile chemical compounds that are utilized as herbal medicines and essential oils. Here, we reported the complete sequencing of the chloroplast genome of Cinnamomum camphora...

We developed a straightforward, rapid, and inexpensive method to determine transgene copy number in tobacco. The plasmid (pSSRCopy) used for tobacco transformation contains a simple sequence repeat (SSR) locus, PT1199, which was partially deleted in the middle, a homogenous SSR locus in tobacco K326. A 168-bp segment of the cloned PT1199 was shortened to 95 bp by deleting a 73-bp internal fragm...

* Author for correspondence: Dr. Maha Abdullah, Immunology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia. Tel.: 603-89472375, Fax: 603-89412787, Email: [email protected]. Abstract. Next-generation sequencing (NGS) technologies, capable of sequencing genomic DNA and RNA at high throughput with unprecedented speed, hav...

The germline chromosomes in Paramecium and other ciliated protozoa contain regions of DNA that are excised and eliminated during the development of a new macronuclear genome. Paramecium tetraurelia internal eliminated sequences (IESs) are invariably flanked by a 5'-TA-3' dinucleotide sequence that is part of a larger 8-bp terminal inverted-repeat consensus sequence. Both features, the absolutel...

Actinidia chinensis is an important economic plant belonging to the basal lineage of the asterids. Availability of a complete Actinidia chloroplast genome sequence is crucial to understanding phylogenetic relationships among major lineages of angiosperms and facilitates kiwifruit genetic improvement. We report here the complete nucleotide sequences of the chloroplast genomes for Actinidia chine...

Introduction: Some segments of the human genome exhibit polymorphism due to a variable number of tandem repeats (VNTR). The enzymatic amplification of VNTR loci can allow for rapid analysis and discrimination of closely sized alleles. We report a PCR-based method for analysis of the highly polymorphic Stl4 VNTR. Source and Description: The Stl4-1 probe is a 3 kb EcoRI fragment cloned from human...

Plastid genomes (plastomes) of angiosperms are well known for their relative stability in size, structure, and gene content. However, little is about heredity variations wide crossing. To such an end, the plastomes five representative rice backcross inbred lines (BILs) developed from crosses O. glaberrima/O. sativa were analyzed. We found that size all was 134,580 bp, with a quadripartite struc...

Indosasa lipoensis, an ornamental garden plant, belongs to the genus of subfamily Bambooaceae within Poaceae. lipoensis is endangered and requires protection owing its relatively narrow distribution area. Chloroplast (cp) genome offers a novel awareness evolutionary genetic variation higher plants. Herein, we assembled elucidated complete cp I. compared it with four previously published genomes...