Immobilization of Carbonic Anhydrase for Biomimetic CO2 Capture Maria Elena Russo, Stefania Scialla, Viviana De Luca, Clemente Capasso, Giuseppe Olivieri, Antonio Marzocchella a Istituto di Ricerche sulla Combustione– Consiglio Nazionale delle Ricerche, P.le V. Tecchio 80, 80125 Napoli, Italy b Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli St...

Citrulline generation in isolated rat liver mitochondria is markedly inhibited by the carbonic anhydrase inhibitor acetazolamide (Dodgson et al., 1983). This finding suggests that hepatic mitochondrial-matrix carbonic anhydrase may exert an important controlling effect on ureogenesis by supplying bicarbonate for carbamoyl phosphate synthesis. Three different carbonic anhydrase isoenzymes have b...

Polyethylene glycol has been shown to bind to the molten globule intermediate on the bovine carbonic anhydrase B folding pathway. The mechanism of this interaction has been extensively probed. Polyethylene glycol (PEG) binds weakly to the molten globule first intermediate as measured by hydrophobic interaction chromatography, but PEG does not bind to either the native state or the second interm...

The branchial epithelium of the mudskipper Periophthalmodon schlosseri is densely packed with mitochondria-rich (MR) cells. This species of mudskipper is also able to eliminate ammonia against large inward gradients and to tolerate extremely high environmental ammonia concentrations. To test whether these branchial MR cells are the sites of active ammonia elimination, we used an immunological a...

To investigate the role of carbonic anhydrase in the recovery of skeletal muscle from anoxia, pH and cell phosphates were measured by (31)P-nuclear magnetic resonance in superfused newborn rabbit myotubes and cultured mouse soleus cells (H-2K(b)-ts a58) after approximately 2-3.5 h without superfusion. In control studies, pH and phosphocreatine fell and P(i) rose during anoxia and recovered with...

Variations in diuretic-mediated inhibition of carbonic anhydrase-dependent chloride transport in platelets and vascular smooth muscle could account for the contrasting efficacy of the thiazide and thiazide-like diuretics in reducing cardiovascular morbidity in patients with hypertension. We assessed platelet carbonic anhydrase activity and catecholamine-induced platelet aggregation in the prese...

The activity of carbonic anhydrase has been estimated by three procedures: manometric (l-3), calorimetric (2, 4-8), and electrometric (9). For investigations of the enzyme concentration of tissues the calorimetric method has often been used in preference to the manometric technique because of its relative simplicity. A disadvantage of the calorimetric method has been the inhibitory effects of t...

OBJECTIVE To report the safe and successful use of the carbonic anhydrase inhibitor acetazolamide for treatment of patients with episodic ataxia and periodic paralysis who had been denied treatment because of a history of severe allergic reactions to antibiotic sulfonamides. DESIGN Case reports. SETTING University of Rochester Medical Center, Rochester, New York. PATIENTS A 61-year-old ma...

Carbonic anhydrases are mostly zinc metalloenzymes that catalyze the reversible hydration/dehydration of CO2/HCO3 (-) Previously, the X-ray crystal structures of CO2-bound holo (zinc-bound) and apo (zinc-free) human carbonic anhydrase IIs (hCA IIs) were captured at high resolution. Here, we present sequential timeframe structures of holo- [T = 0 s (CO2-bound), 50 s, 3 min, 10 min, 25 min, and 1...