background: saccharomyces boulardii (s. boulardii) is the best known probiotic yeast. the genetic engineering of this probiotic strain requires the availability of appropriate mutants to accept various gene constructs carrying different selection markers. as the auxotrophy selection markers are under focus, we have generated a ura3 auxotroph mutant of s. boulardii for use in further genetic man...

Shark cartilage is considered as a natural dietary supplement consisting of anti-angiogenic, immunostimulatory, and anti-inflammatory characteristics. Therefore, this study was designed to inspect the possibility that whitecheek shark (Carcharhinus dussumieri) cartilage proteins motivate expression of NKG2D, CXCR3, NKP46, and NKP44 receptors on natural killer cells and their activities against ...

Blood plasma (BP) is a borderline non-Newtonian fluid. Few studies have characterized the rheology of BP and even less focused on understanding its subtle viscoelastic traits, which were only somewhat recently acknowledged. We use passive microrheology to measure bulk response human samples under shear at body ambient temperatures. Evidence subdiffusive behavior in mean-squared displacement obs...

Thrombolytic stroke therapy with tissue plasminogen activator (tPA) is limited by risks of hemorrhagic transformation (HT). We have reported that a new 12/15-lipoxygenase (12/15-LOX) inhibitor ML351 reduced tPA related HT in mice subjected to experimental under anticoagulation. In this study, we asked whether can ameliorate induced an embolic model. Rats were middle cerebral artery occlusion 2 ...

In order to understand the importance of functional proteins in mosquito behavior, following blood meal, a baseline proteomic dataset is essential for providing insights into the physiology of blood feeding. Therefore, in this study as first step, in solution and 1-D electrophoresis digestion approach combined with tandem mass spectrometry (nano LC-MS/MS) and computational bioinformatics for da...

Background: The study was undertaken to determine the efficiency of supplementation antimicrobial peptide on growth performance, serum biochemistry, intestinal morphology, antioxidant status and gene expression cytokines tight junction proteins in broiler chicks as an alternative antibiotic promoters that impart residues meat. Methods: FCR calculated by dividing respective feed intake weight ga...

Benzimidazoles, an important member of the N-heterocyclic carbenes family, draw attention to their catalytic properties as well pharmaceutical activity. Since these molecules are relatively easy synthesize and derivatize, they frequently used in synthesis species with desired metal complexes species. The interactions kinds tissue blood components important. interaction bioactive serum albumin, ...

Copper (Cu) is a vital trace element for maintaining human health. Current evidence suggests that genes responsible regulating copper influx and detoxification help preserve its homeostasis. Adequate Cu levels sustain normal cardiac blood vessel activity by mitochondrial function. Cuproptosis, unlike other forms of cell death, characterized alterations in enzymes. Therapeutics targeting cupropt...

Malaria sporozoites must invade the salivary glands of mosquitoes for maturation before transmission to vertebrate hosts. The duration of the sporogonic cycle within the mosquitoes ranges from 10 to 21 days depending on the parasite species and temperature. During blood feeding salivary gland proteins are injected into the vertebrate host, along with malaria sporozoites in the case of an infect...

Abstract Background Severe traumatic brain injury (TBI) is associated with blood–brain barrier (BBB) disruption and a subsequent neuroinflammatory process. We aimed to perform multiplex screening of enriched inflammatory proteins in blood cerebrospinal fluid (CSF) order study their role BBB disruption, neuroinflammation long-term functional outcome TBI patients healthy controls. Methods conduct...