background the aim of this study was the ex vivo expansion of umbilical cord blood hematopoietic stem cells on biocompatible nanofiber scaffolds. materials and methods cd133+ hematopoietic stem cells were separated from umbilical cord blood using midimacs (positive selection) system by means of monocolonal antibody cd133 (microbeads) subsequently, flowcytometry method was done to assess the pur...

aim : thereby we aimed to detection of bacterial dna (bactdna) in sbp by polymerase chain reaction (pcr) and its prognostic relevance in cirrhotic patients with culture-negative non-neutrocytic ascites(cnnna).  background: approximately 60% of patients with spontaneous bacterial peritonitis (sbp) are ascites culture negative. methods : of each 77 patients with cirrhosis and ascites, two samples...

objective whatever the health field, compliance with the recommended practice guidelines or parameters for diagnosis by specialists and expert health professionals benefits the patients. this study was conducted to determine the whether or not these guidelines or parameters are applied to the evaluation of children with first simple febrile convulsion (sfc) in a regional teaching hospital. mate...

AND EXPLANATION Blood culture is one of the most important and critical procedures performed in the microbiology laboratory. Since blood is normally sterile, the isolation and identification of an organism has great diagnostic significance. Blood cultures are of great importance in diagnosing such conditions as endocarditis, typhoid fever, pneumonia and other diseases characterized by bacteremi...

BACKGROUND AND OBJECTIVE Blood culture contamination in the pediatric population remains a significant quality and safety issue because false-positive blood cultures lead to unnecessary use of resources and testing. In addition, few studies describe interventions to reduce peripheral blood culture contamination rates in this population. We hypothesized that the introduction of a standardized st...

In early diagnosis of typhoid fever, the emphasis has for long been on the isolation of S. typhi from blood culture. Clot culture techniques have not enjoyed the same vogue, although they are largely used in areas where the disease is endemic. Felix (1924) found that clot culture was hardly ever unsuccessful if blood culture was successful, but that the reverse was frequently true. He attribute...

BACKGROUND Bacterial parapneumonic pleural effusions (PPEs) have high morbidity. The accurate identification of pathogens is vital for initiating the appropriate treatment. A previous study suggested that the use of blood culture bottles might improve the bacterial yield in PPEs. OBJECTIVES The aim of this study was to compare the culture positivity rate by the blood culture bottles and the s...