The gene cassettes found in class 1 integrons are generally promoterless units composed by an open reading frame (ORF), a short 5’ untranslated region (UTR) and a 3’ recombination site ( ). Fused gene cassettes are generated attC by partial or total loss of the from the first cassette in an array, creating, in attC some cases, a fusion with the ORF from the next cassette. These structures are r...

BACKGROUND We have recovered one bla(NDM-1)-harboring bacterial strain, designated as XM1570, from a sputum sample obtained from a fatal case of pneumonia in China. METHODS Biochemical profiling, 16S rRNA sequencing and antimicrobial susceptibility testing were performed. Conjugation experiments were conducted to determine transmissibility of resistance. Pulsed-field gel electrophoresis and w...

*Corresponding author: 17 Teresa Spanu 18 Istituto di Microbiologia 19 Università Cattolica del Sacro Cuore 20 Largo Gemelli, 8 21 00168 – Rome (Italy) 22 Tel: +39 06 30154218; Fax: +39063051152 23 e-mail: [email protected] 24 Copyright © 2012, American Society for Microbiology. All Rights Reserved. J. Clin. Microbiol. doi:10.1128/JCM.00284-12 JCM Accepts, published online ahead of print on ...

INTRODUCTION The emergence and rapid spread of blaIMP and blaVIM metallo-beta-lactamase (MBL) producing Gram-negative bacteria causing nosocomial infections are of concern worldwide due to limited treatment options. METHODOLOGY A total of 179 nonreplicate, consecutive, carbapenem resistant Pseudomonas aeruginosa (61), Acinetobacter baumannii (116), Acinetobacter lwoffii (1) and Pseudomonas st...

BACKGROUND/PURPOSE To investigate the characterization of bla(OxA-23) gene regions in isolates of Acinetobacter baumannii from Taizhou Municipal Hospital. METHODS Fifty-nine non-repetitive, multiresistant (including imipenem-resistant) isolates of A. baumannii were recovered from clinical infections in hospitalized patients from January 2010 to August 2011 in Taizhou Municipal Hospital (affil...

Translational stop codons were introduced at various locations in the protein-coding regions of the monocistronic bla and ompA gene transcripts of Escherichia coli, and the decay characteristics of the upstream and downstream mRNA segments were analyzed. Premature termination of translation at codon position 26 reduced the stability of both the translated and ribosome-free segments of bla mRNA,...

OBJECTIVES Carbapenem-resistant Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa are endemic in many medical centres. Because therapeutic options are limited, understanding the epidemiology and controlling the spread of these pathogens are of paramount importance. METHODS Isolates of K. pneumoniae, A. baumannii and P. aeruginosa were collected from 14 hospitals in New...

244 words 26 Main text: 4376 words 27 . CC-BY 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not . http://dx.doi.org/10.1101/033522 doi: bioRxiv preprint first posted online Dec. 2, 2015;