We read with great interest the editorial by GAKIS [1] about the extreme importance of the adenosine deaminase (ADA) isoenzymes ADA1 and ADA2 on the homeostasis of 2' deoxyadenosine and adenosine, especially when monocytes and macrophages are infected by intracellular microorganisms. Serum ADA activity is increased in various conditions such as liver disease, tuberculosis, typhoid, infective mo...

It is shown here that concurrence between advection and diffusion in a drying sessile drop of a biological fluid can produce spatial redistribution of albumen and salt. The result gives an explanation for the patterns observed in the dried drops of the biological fluids.

The dextran sulphates resemble heparin in being active inhibitors of several different forms of biological activity, e.g., blood coagulation, serum complement activity, and certain enzyme systems (Walton, 1954). In each case the activity of the dextran sulphate appears to be dependent upon direct interaction with one or more protein components of the biological system under investigation. In fa...

The use of several methods to differentiate myoglobin from hemoglobin has been investigated. The immunochemical methods, particularly those of hemagglutination inhibition and radioimmunoassay, are the most useful. This report summarizes work in the Ames Research Laboratory over the past 17 years with the several methods.

Two simple and highly sensitive fluorimetric methods have been developed for the determination of isoxsuprine hydrochloride in bulk, in dosage forms and in biological fluids. The first method involves the direct measurement of the native fluorescence of the drug in the concentration range 0.4-4.0 microg ml(-1), the second method is based on the oxidation of isoxsuprine HCl with cerium(IV) follo...

Results obtained with a potentiometric analyzer, NOVA 1, specific for sodium and potassium, were compared with those by flame photometry. Both instruments showed linearity within a physiological range of sodium and potassium concentrations and had similar precisions. Volume displacements from addition of albumin or Intralipid to aqueous samples yielded the predicted lower flame-photometric resu...

A micro procedure for estimating uric acid in body fluids, based on the carbonate method, is described. Interferences are elucidated. The stoichiometry of the reaction is carefully worked out. Reliability of the method is established by comparison with two other methods, by recovery experiments, and by replicate analyses.

A spectrofluorimetric method for the determination of paracetamol is presented, based on the oxidation of the analyte to give the fluorophore 2,2'-dihydroxy-5,5'-diacetyldiaminebiphenyl. Sodium hypochlorite was used as an oxidizing reagent and the optimum pH was found to be 10.0 (sodium carbonate-boric acid buffer solution). The linear concentration range of application was 0.1-100.0 micrograms...

Research of new diagnosis or prognosis biomarkers is a major challenge for the management of patients with complex pathologies like cancer. Clinical proteomics is one of the recent approaches to identify these biomarkers in biological fluids. Over the last five years, many problems related to the variability and the quality control of these analyses have been observed. This was notably related ...

This paper presents a microfluidic system for helping health professionals with rapid and accurate biological fluids analysis as well as for helping the patient himself at home. This microsystem consists of two wafers: a Pyrex glass wafer containing the microlaboratory (microchannels to carry chemical reagents and sample solutions) and a silicon wafer including the protein detection system (by ...