The effects of detergents on squid giant axon action and resting potentials as well as membrane conductances in the voltage clamp have been studied. Anionic detergents (sodium lauryl sulfate, 0.1 to 1.0 mM; dimethyl benzene sulfonate, 1 to 20 mM, pH 7.6) cause a temporary increase and a later decrease of action potential height and the value of the resting potential. Cationic detergent (cetyl t...

SMA-Lipodisq nanoparticles, with one bacteriorhodopsin (bR) per 12 nm particle on average (protein/lipid molar ratio, 1:172), were prepared without the use of detergents. Using pulsed and continuous wave nitroxide spin label electron paramagnetic resonance, the structural and dynamic integrity of bR was retained when compared with data for bR obtained in the native membrane and in detergents an...

Various detergents can be used to dissolve phospholipids, resulting in very narrow 31PNMR resonances. The resonances are well resolved, allowing identification and quantitative analysis of phospholipids in a mixture. The chemical shift depends strongly on pH, reflecting changes in the state of ionization of the phospholipid headgroup moieties. Samples of phospholipids dissolved in aqueous deter...

background and objectives: sodium dodecyl sulfate (sds) is one of the main surfactant components in detergents and cosmetics, used in high amounts as a detergent in products such as shampoos, car wash soap and toothpaste. therefore, its bioremediation by suitable microorganisms is important. alkylsulfatase is an enzyme that hydrolyses sulfate -ester bonds to give inorganic sulfate and alcohol. ...

One of the several uses of sucrose detergents, as well as other micelle forming detergents, is the solubilization of different membrane proteins. Accurate knowledge of the micelle properties, including size and shape, are needed to optimize the surfactant conditions for protein purification and membrane characterization. We synthesized sucrose esters having different numbers of methylene subuni...

Self-assembly dynamics in binary surfactant mixtures and structure changes of lipid vesicles induced by detergent solution are studied using coarse-grained molecular simulations. Disk-shaped micelles, the bicelles, are stabilized by detergents surrounding the rim of a bilayer disk of lipids. The self-assembled bicelles are considerably smaller than bicelles formed from vesicle rupture, and thei...

Solubilized proteins Detergent-solubilization of membrane proteins has proved to be a successful approach in many cases. Both ionic and nonionic detergents have been used: the choice of detergent may be crucial for retaining protein activity. Generally, detergents should be used at concentrations above their critical micelle concentration (CMC). Micelles formed above the CMC mimic a membrane en...

The effect of detergents on the activity of palmityl coenzyme A: carnitine 0-palmityltransferase (EC 2.3.1.-) has been investigated. A series of detergents (n-palmitylcarnitine, palmitylcholine, caprinylcholine, free fatty acids, deoxycholate, digitonin, Tween 20, Tween 80, and Triton X-100) have been found to stimulate the incorporation of L-carnitine-CH@H into L-palmitylcarnitine in the prese...