FP is based on the principle that when a small fluorescent molecule (the tracer) is excited with plane-polarized light, the emitted light is largely depolarized because the molecule rotates rapidly in solution during its fluorescence lifetime. If the tracer is bound to a large molecule, thereby increasing its molecular volume, its rotation is slowed and the emitted light remains in the same pla...

A LightCycler real-time PCR hybridization probe-based assay which detects a partial Klebsiella pneumoniae 16S rRNA gene was developed for the rapid identification of K. pneumoniae directly from growth-positive blood culture bottles (BACTEC 9240 system) within 2 h. No cross-reactivity was observed with 65 negative-control blood cultures that grew bacteria other than K. pneumoniae and 48 negative...

The data presented in this article are related to the research article entitled "Epoxide hydrolase of Trichoderma reesei: Biochemical properties and conformational characterization" [1]. Epoxide hydrolases (EHs) are enzymes that catalyze the hydrolysis of epoxides to the corresponding vicinal diols. This article describes the optimal parameters for the colorimetric red assay to determine the en...

CLINICAL CHEMISTRY, Vol. 30, No. 10, 1984 1715 Clinical Physiology. Recommendedmethod for the determinationof creatine kinase in blood modified by the inclusionof EDTA. Ibid. 39, 1-5 (1979). 4. Balkcom RM, Amano E. Adenylate kinase inhibition by creatine phosphatein CK assay. Clin Chem 24, 1289 (1978). Letter. 5. Bergmeyer JU, Gawehn K, Grasal M. Enzymes as biochemical reagents. In Methods of E...

Biochemical reaction systems may be viewed as discrete event processes characterized by a number of states and state transitions. These systems may be modeled as state transition systems with transitions representing individual reaction events. Since they often involve a large number of interactions, it can be difficult to construct such a model for a system, and since the resulting state-level...

An integrated strategy that combined in silico screening and tiered biochemical assays (enzymatic, in vitro, and ex vivo) was used to identify and characterize effective small-molecule inhibitors of Clostridium botulinum neurotoxin serotype A (BoNT/A). Virtual screening was initially performed by computationally docking compounds of the National Cancer Institute (NCI) database into the active s...

The bottle bioassay measuring the time-mortality rate is a simplified procedure for detecting insecticide resistance. It can be used with a biochemical microplate assay to identify the mechanism involved. This integrated approach was used to detect temephos resistance in Aedes aegypti from Nonthaburi (lowest use) and Roi Et (highest use). Ae. aegypti BKK1 laboratory strain was used as the susce...