Biodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is effective biocatalyst for the bioprocess under conventional growing conditions. In present work, a novel composed by R. resting cells evaluated first time. Fluid dynamic conditions has been optimized maxi...

Galactose oxidase from Fusarium graminearum IMV-1060 adsorbed on, and covalently bound to, silica carriers has been used for analytical determinations of D-galactose and galactose-containing sugars. Using a flowing oxygen electrode of the Clark-type, sensor system for enzymatic analysis of water solutions of galactose-containing carbohydrates was made. Measurements were taken both in the pulse ...

Different enantiopure terminal epoxides or bromohydrins have chemoselectively been synthesised in one-pot starting from the corresponding α-bromoketones through alcohol dehydrogenase (ADH)-catalysed processes adding an organic co-solvent and tuning appropriately the medium pH and the temperature. Thus, at neutral pH enantiopure bromohydrins were obtained while using basic conditions (pH 9.5-10)...

The term ‘Enzyme’ came from the Greek word ‘Enzymos’ means ‘In the cells or Ferments’. These are naturally available, high molecular weight proteins, capable of catalyzing chemical reaction of biological process and therefore popular as ‘Biocatalyst’. Enzymes are soluble organic substance, produced by living organisms whose different amino acids form long poly peptide chains (-HN-R-CONH-R-CO-)n...

Enzymes that catalyze carbon-carbon bond formation can be exploited as biocatalyst for synthetic organic chemistry. However, natural enzymes frequently do not possess the required properties or specificities to catalyze industrially useful transformations. This mini-review describes recent work using knowledge-guided site-specific mutagenesis of key active site residues to alter substrate speci...

We reported the first enzyme-catalysed, direct, three-component asymmetric Mannich reaction using protease type XIV from Streptomyces griseus (SGP) in acetonitrile. Yields of up to 92% with enantioselectivities of up to 88% e.e. and diastereoselectivities of up to 92:8 (syn:anti) were achieved under the optimised conditions. This enzyme's catalytic promiscuity expands the application of this bi...

We present a re-usable enzyme catalyst system via direct encapsulation of cellobiase in nonsurfactant templated sol–gel mesoporous silica host material with D-fructose as the template. The pore diameter and porosity of the silica host material, controlled by the fructose content, controlled the diffusion of substrate to the enzyme. This in situ immobilized cellobiase showed little or no leakage...

A microbial desalination cell (MDC) built on a modified surface has been studied for seawater desalination. The goal of this study is to provide and develop system that does not require energy support by applying modification the anode as an electron acceptor. different potential charges occur between cathode can serve driving force electrodialysis seawater, resulting in its Yeast applied bioca...

A nanolayer of composite and Rhodococcus erythropolis biocatalyst was studied for the first time for desulfurization of dibenzothiophene as a model sulfur compound and its performance was compared with that of composite and R. erythropolis alone. The nanolayer of composite was synthesized by sol-gel method from ferrous oxalate and zinc oxalate precursors coated on glass by spin coating techniqu...

A theoretical and experimental study was carried out on the biocatalytic production of babassu biodiesel through enzymatic hydroesterification. The complete hydrolysis oil using a 1:1 mass solution at 40 °C for 4 h 0.4% lipase from Thermomyces lanuginosus (TLL). Then, with use Eversa® Transform 2.0 in esterification step, statistical design used, varying temperature (25–55 °C), molar ratio betw...