According to Newton’s law of gravity, two masses interact with each other via a central force which can be derived from the potential VN (r) = −Gm1m2/r where G is a universal constant known as Newton’s constant. Likewise, according to Coulomb’s law, two electrically charged particles with charges n1e and n2e interact via the central potential VCb (r) = αn1n2/r where e is the elementary charge a...

one of the important parts of innate immunity is complement system that occurs in three different ways; the classic, the alternative and the lectin pathway. the four pattern recognition molecules that have been identified till now are mannose binding lectin (mbl), a component of lectin pathway, and three ficolins (ficolin1,-2 and -3) which compound to the carbohydrates of the cell surface. mbl ...

Protein specific aptamers are highly applicable affinity ligands in different fields of research and clinical applications. They have been developed against various targets, in particular, bio-macromolecules such as proteins. Among human proteins, the coagulation factors are the most attractive targets for aptamer selection and their specific aptamers had valuable characteristics in therapeutic...

In this study, the effect of temperature on the diffusion welding of AZ31 magnesium alloy and 7075 aluminum was discussed. Diffusion welding temperature was the most important parameter since all mechanisms are sensitive to temperature in Diffusion welding method. Intrusive contacts between the two alloys at temperatures of 430, and 440 °C 450 and took 60 min. In all tests constant pressure and...

Binding sites for rat atrial natriuretic peptide (6-33) were quantified by incubation of brain sections with (3-[125I]iodotyrosyl28) rat atrial natriuretic peptide (6-33), followed by autoradiography with computerized microdensitometry. Spontaneously hypertensive rats present lower numbers and lower affinity of binding sites than normotensive controls, Wistar-Kyoto rats, in the subfornical orga...

An enzymatic assay is described for non-albuminbound bilirubin in the serum of newborn infants. Unbound bilirubin is oxidized to colorless compounds by ethyl hydroperoxide in the presence of horseradish peroxidase (EC 1.11.1.7), while albumin-bound bilirubin is protected from oxidation. Because the equilibrium between albumin and bilirubin occurs rapidly, the oxidation step is rate limiting, an...

The DNA binding property of 5,10,15-Tris(4-carboxyphenyl) corrole (TCPC) was studied by UV-Visible, fluorescence and circular dichroism (CD) spectroscopic methods. TCPC can bind to ct-DNA via an outside binding mode with the binding constant of Kb = 1.05 × 10⁵ M(-1). TCPC also displayed good photonuclease activity, which involves singlet oxygen species (¹O₂). The binding constant between TCPC a...

Differential scanning calorimetric experiments show that addition of Al(III) to conalbumin increases its denaturation temperature by 5 degrees, from 60 to 68 degrees. Only one Al(III) bound per conalbumin molecule produces this change in heat stability; additional bound Al(III) does not affect the heat stability. Since Al(III) displaces both Cu(II) bound at the metal binding sites of conalbumin...

The interaction of proflavine with herring sperm DNA has been investigated by cyclic voltammetry and UV-Vis spectroscopy as well as viscosity measurements. Shifts in the peak potentials in cyclic voltammetry, spectral changes in UV absorption titration, an increase in viscosity of DNA and the results of the effect of ionic strength on the binding constant strongly support the intercalation of p...

3 H-Spiroperidol, a butyrophenone antipsychotic agent, binds specifically and with high affinity to neuroleptic receptors in the 3 corpus striatum and substantia nigra. Scatchard analysis of Hspiroperidol isotherms in the rat striatum revealed a dissociation constant (KQ) of 30 pM and a total receptor density (Bmax) of 430 fmoles/mg protein. In the substantia nigra, the and Bmax values were 42 ...