The D-Ala-D-Ala carboxypeptidases/transpeptidases (penicillin-binding proteins, PBPs) share considerable structural homology with class A beta-lactamases (EC 3.5.2.6), although these beta-lactamases have no observable D-Ala-D-Ala carboxypeptidase activity. With the objective of recruiting such activity into a beta-lactamase background, we have prepared a chimeric protein by inserting a 28-amino...

OBJECTIVES To characterize the AmpC-type beta-lactamases produced by Escherichia coli clinical isolates. METHODS E. coli isolates recovered in a French hospital in 2006 were selected on the basis of a resistance phenotype consistent with increased AmpC production. The presence of genes coding for plasmid-mediated cephalosporinases as well as the existence of mutations in the chromosome-borne ...

BACKGROUND & OBJECTIVE The production of carbapenemases is an important mechanism responsible for the carbapenem resistance. A simple and inexpensive testing method for screening of carbapenemase producers is essential. A prospective study was undertaken to detect metallo-beta-lactamases (MBLs) and AmpC beta-lactamases in nonfermentative Gram negative bacteria and to evaluate the various method...

Over the years, antibiotics have been among primary drugs used to treat or prevent infections in animals and humans. ESBL AmpC producing isolates become common microorganisms medical institutions. They contributed immensely clinical challenges caused by nosocomial with minimal therapeutic options.The present study investigated prevalence of extended-spectrum beta-lactamases Ambler class C Enugu...

Objective(s) Resistance to the new generation of cephalosporins which is mediated by Extended-Spectrum beta-lactamases (ESBLs) has been found among Escherichia coli isolates throughout the world. These resistance genes and their producers, the micro-organisms carrying beta-lactamases, are responsible for serious clinical and therapeutic problems among inpatients and it is necessary to pay more...

in modern medical processes, new antimicrobial drugs are being widely used in order to rapid and essential eradication of resistant bacterial strains. broad-spectrum beta-lactamases are enzymes originated from mutations in the common plasmid genes mediators for beta-lactamases such as tem-1, tem-2, and shv-1 are transferred among bacterial species. the genes coding the production of esbl are of...

Nicotinamide phosphoribosyl-transferases (NAMPT) are enzymes that play a role in targeting cancer metabolism, while beta lactamases are involved in bacterial resistance to beta-lactam antibiotics. Many protein inhibitors exhibit such property which is often correlated with their cellular potency. In order to understand such a phenomenon, the present article conducts an analysis of the dynamic b...

Cephabacin F group antibiotics with a 7-formylamino substituent showed antibacterial activity against a wide variety of bacteria including beta-lactamase-producing clinical isolates and anaerobic bacteria. Cephabacin H group antibiotics without the substituent showed more potent activity against Gram-positive bacteria than cephabacin F group antibiotics, but were not active against Gram-negativ...