BACKGROUND Numerous strategies have been developed for the display of heterologous proteins in the surface of live bacterial carriers, which can be used as vaccines, immune-modulators, cancer therapy or bioremediation. Bacterial biofilms have emerged as an interesting approach for the expression of proteins of interest. Bacillus subtilis is a well-described, endospore-forming organism that is a...

Surface display is a powerful technique that uses natural microbial functional components to express proteins or peptides on the cell exterior. Since the reporting of the first surface-display system in the mid-1980s, a variety of new systems have been reported for yeast, Gram-positive and Gram-negative bacteria. Non-conventional display methods are emerging, eliminating the generation of genet...

We describe an efficient approach to rendering a perspectively correct image on a potentially irregular display surface that may be illuminated with one or more distinct devices. The first pass of the technique generates an image of the desired graphics model using conventional rendering. The second pass projects that image as a texture onto a model of the display surface, then re-renders the t...

Front-projection display systems suffer from a fundamental problem: the user can easily and inadvertently block the projector, creating shadows. The framework we present in this paper enables a front-projected display system to solve this problem and remove display surface shadows in real-time. Shadows are an example of radiometric inconsistencies, which cause a number of challenges in building...

In this study, the TiO2 nanorods were synthesized from P25 TiO2 nanoparticles by hydrothermal method in 10 M NaOH solution. The effects of annealing temperatures on produced nanorods were investigated  by scanning  electron microscopy (SEM) X-ray diffraction (XRD) and photoluminescence (PL) spectroscopy. Diameter growth...

We describe a phage display technique that allows the production and selective enrichment of phages that display an N-glycoprotein (glycophages). We applied glycophage display to select functional glycosylation sequons from a pool of randomized acceptor sequences. Our system provides a genetic platform to study and engineer different steps in the pathway of bacterial N-linked protein glycosylat...