Background: Interferon-gamma [IFN-γ) is the most important cytokine in immune system. This protein has been expressed bacterial cells. However, cloning not an easy task. We aimed to clone, express, and purify recombinant mouse IFN-γ overcome problems favor of commercial purposes. Materials Methods: To amplify gene product for cloning, we primarily designed two specific primers target gene. Foll...

Class III peroxidases (CIII Prxs) play critical roles in plant immunity by scavenging reactive oxygen species (ROS). However, the functions of CIII Prxs rice (Oryza sativa L.) are largely unexplored. Here, we report a Prx precursor, OsPrx30, that is responsive to bacterial blight Xanthomonas oryzae pv. (Xoo). OsPrx30 was primarily expressed roots, leaves, and stems, its protein product mainly l...

Among vegetable functional products, soy-based foods stand out for their high content in protein and flavonoids, mainly isoflavones. The fermentation of soy beverages with selected bacterial strains is a way to obtain product enriched isoflavone aglycones, alongside other derivates bioactive flavonoids. Two activity on the transformation these compounds, Limosilactobacillus mucosae INIA P508 Bi...

serological methods are commonly used methods for detection of viruses. preparation of pure viral antigens is a crucial step in production of antibodies required for serological studies. in this research the gene encoding coat protein of a beet western yellows virus (bwyv) isolate from iran was amplified by pcr and was ligated into a bacterial expression vector (pet26b) to obtain pet-bwyv-cp cl...

Blood is the main by-product from slaughterhouses bearing high levels of suspended solids (18% w/w) and BOD5 (250 000–375 000 mg O2/L), which makes difficult its handling disposal. This study proposes valorisation blood protein to produce enzymatic hydrolysates rich in haemic iron antioxidant peptides. Haemic presents higher bioavailability compared inorganic form, but incorporation into foodst...

Optimal gene expression is central for the development of both bacterial systems heterologous protein production, and microbial cell factories industrial metabolite production. Our goal to fulfill industry-level overproduction demands optimally, as measured by following key performance metrics: titer, productivity rate, yield (TRY). Here we use a multiscale model incorporating dynamics (i) popu...

  The objective of the current study was to investigate the effects of whey protein coating on the quality of common Kilka during frozen storage. For this study, common Kilka was coated with 20% whey protein concentration. Non- coated Kilka also was used as a control. Coated and non-coated samples were then stored at -18 °C for six months. Bacterial, chemical and sensory properties of the sampl...