OBJECTIVE To evaluate the effects of single- and double-spin preparations of platelet-rich plasma (PRP) on anabolic and catabolic activities of cartilage and meniscal explants in vitro. METHODS Single- and double-spin PRP was prepared using laboratory processing or commercial kits. The cellular contents were quantified, and each PRP was mixed in equal quantities with cell culture medium and a...

INTRODUCTION Platelet-rich plasma (PRP) is a fraction of plasma in which several growth factors are concentrated at high levels. The active soluble releasate isolated following platelet activation of PRP (PRP-releasate) has been demonstrated to stimulate the metabolism of IVD cells in vitro. The in vivo effect of PRP-releasate on degenerated IVD remains unknown. The purpose of this study was to...

INTRODUCTION: Platelet-rich plasma (PRP) belongs to one plasma division isolated by centrifugation of whole blood. From 1981 to 1991, a number of different growth factors containing platelets were reported one after another, such as platelet-derived growth factor (PDGF), transforming growth factor beta 1 (TGF-b1), transforming growth factor beta 2 (TGFb2), insulin-like growth factor (IGF), epid...

Purpose: The aim of this study was to compare a new method for the production of platelet-rich plasma (PRP), the plasma-rich-in-growth-factors kit (PRGF kit; G.A.C. Medicale San Antonio, Vitoria, Spain), with an established method, the Platelet Concentrate Collection System (PCCS; 3i/Implant Innovations, Palm Beach Gardens, FL) with respect to resulting cellular and growth factor contents. Mate...

objective(s): platelet-rich plasma (prp) has recently emerged as a promising strategy in regenerative medicine due to its multiple endogenous growth factors. little is known about the role of prp as a promoter in chondrogenesis of human adipose derived stem cells (hadscs). the aim of this study was to determine whether prp may be considered as a natural and easy achievable source of growth fact...

Objective: To compare mean resting pain relief and passive external rotation improvement by Intra-articular Steroid versus intra-articular Platelet Rich Plasma injection in patients with adhesive capsulitis.
 Study Design: Prospective comparative study.
 Place Duration of Study: Armed Forces Institute Rehabilitation Medicine, Rawalpindi Pakistan, from Jan to Jun 2019.
 Methodolog...

This study evaluated the quality and bacteriologic safety of platelet-rich plasma (PRP) produced by 3 simple, inexpensive tube centrifugation methods and a commercial system. Citrated equine blood collected from 26 normal horses was processed by 4 methods: blood collection tubes centrifuged at 1200 and 2000 × g, 50-mL conical tube, and a commercial system. White blood cell (WBC), red blood cell...

Tohidnezhad, M; Wruck, C.J.; Varoga, D; Lippross, S; Drescher, W; +Pufe, T + Department of Anatomy and Cell Biology, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, Department of Trauma Surgery, UKSH Campus Kiel, Department of Orthopaedic Surgery, RWTH Aachen University [email protected] and [email protected] Introduction Open fractures of bone are often colonized by several bacteri...

Background Adipose-derived stem cells (ASCs) are a powerful tool for cosmetic surgery and regenerative medicine. The use of autologous platelet rich plasma (PRP), particularly in combination with ASC-based therapy, has significantly expanded in recent years. Unfortunately, the mechanisms and optimal dosing responsible for the beneficial effects of PRP remain poorly understood. Here we investiga...

OBJECTIVE (1) To compare the performance of 4 platelet counting technologies in equine platelet concentrates and (2) to evaluate the ability of the Magellan platelet rich plasma (PRP) system to concentrate equine platelets. STUDY DESIGN Experimental study to assess method agreement. ANIMALS Adult mixed breed horses (n = 32). METHODS Acid citrate dextrose-A anti-coagulated whole blood was ...