as part of a series of investigations at baba baghi leprosarium in lran, 44 long-treated leprosy patients were selected for our study. samples of early morning sputum were obtained from each patient, examined by microscopy for acid-fast bacilli (afb), and cultured for tubercle bacilli. these tests were negative, but the polymerase chain reaction (pcr) for an insertion sequence believed to be sp...

background: lower respiratory tract infections are among important causes of morbidity and mortality for all age groups. the emergence of multidrug resistant gram-negative bacilli is an issue of increasing concern. materials and methods: a retrospective study including respiratory specimens (sputum and bal) was conducted in our tertiary care centre. samples were processed for microscopy, cultur...

Whilst endeavouring to discover the cause of an outbreak of diphtheria in the girls' medical ward of the Children's hospital, it occurred to me that it was within the bounds of Possibility that the disease had been introduced by a patient, suffering from some other complaint, who had still active bacilli present in the throat or nose after some previous attack diphtheria. This opinion was subse...

prevailed among the villagers, and I proceeded to Sripur to investigate matters. I found four further cases who were still suffering from malignant pustules, the pustules having broken down and forming the characteristic ulcers, as shewn in the illustration on p. 23. The ulcer on the back of the adult female patient had given rise to much sloughing and had exposed the tendons. Films and scrapin...

The presence of acid fast bacilli in sputum specimen was investigated comparatively with Ziehl-Neelsen (ZN)and Kinyoun Cold staining (KC) in order to determine sensitivity in detecting Tuberculosis Bacilli (T.B.). This study was employed on patients with severe lower respiratory tract infection to compare between these two stains. Which revealed that Ziehl-Neelsen procedure gives better results...

The culture and biochemical features of 10 non-chromogenic variants of S. marcescens isolated from clinical sources are reported. Autoagglutination titres were significantly high in sera from those patients in whom Serratia bacilli appeared to contribute to the illness. Since the taxonomic stature of the Serratia bacilli is not clear it might be better to limit the identification of this group ...

Emergence of the newly identified 16S rRNA methylases RmtA, RmtB, and ArmA in pathogenic gram-negative bacilli has been a growing concern. ArmA, which had been identified exclusively in Europe, was also found in several gram-negative pathogenic bacilli isolated in Japan, suggesting global dissemination of hazardous multiple aminoglycoside resistance genes.

If tubercle bacilli could be easily detected in clinical samples with gram staining, it would be possible to detect tuberculosis more promptly and easily, which may contribute to tuberculosis control. In this paper, we present three infective tuberculosis cases in which gram staining easily detected tubercle bacilli before Ziehl-Neelsen (Z-N) staining and diagnosed by polymerase chain reaction ...

1. Rabbits vaccinated with tubercle bacilli killed by exposure to formalin (0.4 per cent) did not show any acquired resistance to subsequent infection with bovine tubercle bacilli, while rabbits vaccinated with tubercle bacilli which had been killed by heating to 70 degrees for 1 hour survived more than half as long again as their controls. 2. Intraperitoneal injection of either the formalin-ki...

Abstract Background The aim of this study was to compare the performance new flow cytometer UF-5000 with UF-1000i and Gram staining for determining bacterial patterns in urine samples. Methods Women who attended our clinic symptoms suggestive urinary tract infection were enrolled study. Mid-stream samples collected gram staining, analysis cultures. Bacterial classified using (none, cocci bacter...