Loop-mediated isothermal amplification (LAMP) is a rapid method with high specificity and efficiency under isothermal condition using a set of four specifically designed primers that recognize six distinct sequences on the target gene. In this study, a LAMP method was developed for specific detection of Babesia orientalis in water buffalo (Bubalus babalis, Linnaeus, 1758). Four primers were des...

Bovine babesiosis and other tick borne diseases are considered responsible for more than 50% losses in the crossbred cattle. Carrier cattle infected with babesiosis are challenge to current diagnostic methods and are difficult to detect because of the low number of parasites in circulation. However, diagnosis of carrier animals in herd is important for preventing outbreaks by transmission throu...

studies on the pathogenesis of babesia ovis infection following blood transfusion of infected blood to sheep with intact spleen and splenectomised sheep showed that all animals developed fever concurrent with a parasitaemia that were occurred within 2-4 days post-inoculation (dpi), clinical signs of disease were severe and included varying degrees of anorexia, listlessness, anaemia, moderate ja...

Deger Y., H. Mert, S. Dede, F. Yur, N. Mert: Serum Total and Lipid-Bound Sialic Acid Concentrations in Sheep with Natural Babesiosis. Acta Vet. Brno 2007, 76: 379-382. The aim of this study was to determine the serum concentrations of total and lipid-bound sialic acid in sheep naturally infected with Babesia ovis before and after treatment. Thirty diseased sheep and ten control animals were use...

A COMPARATIVE STUDY OF AN ELISA TEST AND AN INDIRECT IMMUNOFLUORESCENCE TEST FOR SEROLOGICAL DIAGNOSIS OF BABESIA BOVIS INFECTION. Detection of antibodies to Babesia bovis in cattle is essential for the understanding of the epidemiology of babesiosis and this study was concerned with comparing the indirect fluorescent antibody with the ELISA. Both assays gave rise to 100% sensitivity whilst the...

equine piroplasmosis is a tickborne disease of equids with worldwide distribution, caused by theileria equi and babesia caballi. the aim of this study was molecular detection of t. equi and b. caballi in donkeys in northeastern iran and investigate the association between positivity of piroplasm infection and host-related factors. in the present study, blood samples were collected from 106 appa...