infectious bursal disease virus (ibdv) is the causative agent of gumboro disease, an infectious disease of global economic importance in poultry. structural protein vp2 of ibdv is the most frequently studied protein due to its significant roles in virus attachment, protective immunity, and serotype specificity. the objective of the present study was to improve the expression of hypervariable re...

conclusions these results showed enhanced muscle atrophy processes following peripheral nerve damage and might provide a useful approach to study underlying muscle mechanisms associated with clinical neuropathic pain syndromes. results compared to control animals, l5 ligated animals developed mechanical and heat hypersensitivity during total period of study. soleus muscle weight as well as cdk5...

melamine is a potentially hazardous compound and one of the major concerns especially in dairy products and pet foods. in the present study a sensitive, simple and reliable method for extraction and determination of melamine in infant milk and coffee mate has been developed. this method consists of an initial extraction in buffer media prepared by formic acid and sodium formate, followed by pro...

in this study theeffect ofdrought stress was investigated on grain yield and oil rate and protein percentage of four varieties of castor in the climatic conditions of damghan. the experiment was done in the research farm of damghan islamic azad university(iran)in 2011 assplit plots in a randomized complete block design with three replications. the main plots of drought stress were 5, 10 and15 d...

background microsatellites have evoked the interest of researchers owing to their applications in different fields such as dna fingerprinting, genetic mapping, population genetics, forensics, paternity studies and evolution. objectives the present study focused on the analysis of simple sequence repeats (ssrs) in genomes of seven species from three genera of the filoviridae family. materials an...

introduction : surgical repair of cleft palate with a bony bridge may offer some distinct advantages including a lower rate of fistula formation. the goal of this study was to evaluate the feasibility of surgically inducing cleft palate and reconstruction of the cleft with the use of bone morphogenetic protein-2 (bmp-2) in sheep. materials and methods : in the control group (two sheep), the cle...

introduction: in recent years, influenza viruses have caused moderate to severe infections all around the world while so far there is no influenza vaccine that can protect people with only one dose of injection. in this regard, producing a universal vaccine based on virus-like-particles (vlp) could be an ideal approach.  methods: in this study, the full-length orf of influenza hemagglutinin (ha...

results cloning was confirmed by pcr and restriction digestion. rt-pcr and sds-page represented expression of kgf in e. coli. the optimized expression was achieved 16 hours after induction with 0.3 mm iptg at 37°c in luria broth (lb) containing kanamycin. the 18 kda protein was confirmed by western blotting, using anti-his antibodies. conclusions the result of the present study indicated that e...

interference with microtubule polymerization results in cell cycle arrest leading to cell death. colchicine is a well-known microtubule polymerization inhibitor which does so by binding to a specific site on tubulin. a set of 3',4'-bis (substituted phenyl)-4'h-spiro[indene-2,5'-isoxazol]-1(3h)-one derivatives with known antiproliferative activities were evaluated for their t...