Background: Since ranitidine inhibits the activity of testis Na+-K+ATPase, Ca++ATPase and Mg++ATPase enzymes, and affects estrogen metabolism in the liver and causes gynecomastia, the effects of long term use of ranitidine on testis were studied. Materials and Methods: In this experimental study 40 two-month aged male rats were divided into four groups (control group and 1, 2&3 test groups). T...

The effect of Annona squamosa, Lawsonia inermis and their combination with other herbal molluscicides were studied on different enzyme activity in the nervous tissue of Lymnaea acuminata. Twenty-Four hour in vivo exposure to 40% and 80% of 24 h LC50 of plant derived molluscicides and their combination with other molluscicides such as Cedrus deodara, Azadirachta indica oil, Allium sativum, Polia...

3‘-0-(4-Benzoyl)benzoyl ATP (BzATP) was synthesized and used as a photoactivatable, covalently binding affinity probe to study site-specific adenine nucleotide binding to the ATPase of submitochondrial particles and the purified soluble F1-ATPase of rat liver mitochondria. In the absence of actinic light, BzATP was a good substrate for enzymic hydrolysis with both soluble and membrane-bound F1-...

The polarized distribution of Na,K-ATPase at the basolateral membranes of renal tubule epithelial cells is maintained via a tethering interaction with the underlying spectrinankyrin cytoskeleton. In this study, we have explored the mechanism underlying the loss of Na,K-ATPase polarity following ischemic injury in MDCK cells, utilizing a novel antibody raised against a recently-described kidney-...

The therapeutic effect of cardiac glycosides for congestive heart failure lies in their reversible inhibition on Na+, K+-ATPase located in human myocardium. Several steroid-like compounds containing a core structure similar to cardiac glycosides have been found in many Chinese herbs and medicinal animal products conventionally used to promote blood circulation. They are putatively responsible f...

The Pma1 H -ATPase of Saccharomyces cerevisiae, which functions physiologically to pump protons out of the cell, is one of the most abundant proteins in the yeast plasma membrane (1). It is a 100-kDa polypeptide, anchored in the membrane by 10 hydrophobic -helices (Fig. 1) (2) and belonging to a widespread family of cation transporters known as the P2-type ATPases (6). Members of the P2 family ...

Congenital polycystic kidney disease is characterized by the formation of large fluid-filled cysts in kidney tubules. It has been postulated that increased epithelial cell proliferation and altered transtubular fluid transport are necessary for cyst formation. To address the latter problem, we have studied the plasma membrane distribution of the alpha 1 and beta 1 subunits of Na+/K(+)-ATPase du...

TO THE EDITOR: I would like to congratulate Adriaensen and colleagues for their excellent works that contribute to the detailed morphology of neuroepithelial bodies (NEBs) (1–3). They form a structural basis for understanding NEB physiology. A very interesting thought proposed by the authors is that NEBs may be mechanosensors. To examine this issue, we injected flourescent dye into the nodose g...