A non-invasive method to characterize human mesenchymal stromal cells during adipogenic differentiation was developed for the first time. Seven fatty acid methyl esters (FAMEs), including methyl laurate, methyl myristate, methyl palmitate, methyl linoleate, methyl oleate, methyl elaidate and methyl stearate, were used for characterizing adipogenic differentiation using headspace solid-phase mic...

A novel approach based on headspace solid-phase microextraction (HS-SPME) combined with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-ToFMS) was developed for the simultaneous screening of microbial and mite contamination level in cereals and coffee beans. The proposed approach emerges as a powerful tool for the rapid assessment of the microbial contam...

A highly sensitive and selective method has been developed for the determination of methylmercury in biological specimens and NIST Standard Reference Materials (SRMs). The procedure involves microwave extraction with acetic acid, followed by derivatization and headspace solid-phase microextraction (SPME) with a polydimethylsiloxane (PDMS)-coated silica fiber. Optimization of conditions includin...

The development of a simple and rapid multielemental speciation method is described with the ultimate goal to simultaneously determine various organometallic compounds of mercury, lead and tin (inorganic mercury, methylmercury, trimethyllead, triethyllead, monobutyl-, dibutyl- and tributyltin) in natural water samples. The analytical method consists on the ethylation with NaBEt4, simultaneous h...

Many volatile compounds generated during the thermal processing of milk have been associated with cooked, stale, and sulfurous notes in milk and are considered as off-flavor by most consumers. A headspace solid-phase microextraction (HS-SPME)/gas chromatographic technique for the quantitative analysis of thermally derived off-flavor compounds was developed in this study. The extraction temperat...

Substances classified as unknown or variable composition, complex reaction products biological origin (UVCB) present a challenge for environmental hazard and risk assessment. Here, we novel approach whole-substance bioconcentration testing applied to cedarwood oil—an essential oil composed of volatile, hydrophobic organic chemicals. The method yields whole-body elimination rate constants mixtur...

An optimum method has been developed for extracting volatile organic compounds (VOCs) which contribute to the aroma of different species of citrus fruit (orange, lemon, lime, and mandarin). Headspace solid phase microextraction (HS-SPME) combined with gas chromatography (GC) coupled with flame ionization detection (FID) is used as a very simple, efficient and nondestructive extraction method. A...

The GC and GC/MS analyses of the solvent organic extractive from the stomach of the bees, having collected Mentha spp. nectar, revealed the presence of methyl syringate (6.6%), terpendiol I (5.0%) and vomifoliol (3.0%) that can be attributed to the plant origin. Other major compounds from the bee-stomach were related to the composition of cuticular waxes and less to pheromones. Organic extracti...

Volatile organic compounds of Amorpha fruticosa honey samples were isolated by headspace solid-phase microextraction (HS-SPME) and ultrasonic solvent extraction (USE), followed by gas chromatography and mass spectrometry analyses (GC, GC-MS), in order to obtain complementary data for overall characterization of the honey aroma. The headspace of the honey was dominated by 2-phenylethanol (38.3-5...

A simple, rapid and accurate method on the basis of solid phase microextraction (SPME) in combination with multicapillary gas chromatography (MCGC) hyphenated to inductively coupled plasma-time-of-flight mass spectrometry (ICP-TOFMS) was developed for simultaneous speciation analysis of 10 organometallic compounds of mercury (including inorganic mercury), tin and lead. Headspace SPME was used f...