The artificial WSTF PHD_EL5 RING finger was designed via "α-helical region substitution", and its structural model for the attachment of activated ubiquitin has been demonstrated. Chemical modifications of Cys residues, the circular dichroism spectra, and substrate-independent ubiquitination assays illustrated that the WSTF PHD_EL5 RING finger has E3 activity, and it is ubiquitinated via Lys14....

An artificial RNA ligase specific to RNA with a 5'-triphosphate (PPP-RNA) exhibits broad sequence specificity on model substrates and secondary siRNAs with direct applications in the identification of PPP-RNAs through sequencing.

Artificial Neural Network (ANNs) techniques are recently indicating a lot of promises in the application of various micro-engineering fields. Such a use of ANNs for estimating the patch dimensions of a microstrip line feed rectangular microstrip patch antennas has been presented in this paper. An ANN model has been developed and tested for rectangular patch antenna design. The performance of th...

We report on our efforts to create efficient artificial metalloenzymes for the enantioselective hydrogenation of N-protected dehydroamino acids using either avidin or streptavidin as host proteins. Introduction of chiral amino acid spacers – phenylalanine or proline – between the biotin anchor and the flexible aminodiphosphine moiety 1, combined with saturation mutagenesis at position S112X of ...

The rapid worldwide growth of offshore renewable energy production will provide marine organisms with new hard substrate for colonization, thus acting as artificial reefs. The artificial reef effect is important when constructing, for example, scour protections since it can generate an enhanced habitat. Specifically, artificial structures can create increased heterogeneity in the area important...

Enzymes catalyze organic transformations with exquisite levels of selectivity, including chemoselectivity, stereoselectivity, and substrate selectivity, but the types of reactions catalyzed by enzymes are more limited than those of chemical catalysts. Thus, the convergence of chemical catalysis and biocatalysis can enable enzymatic systems to catalyze abiological reactions with high selectivity...

The objective of this investigation was to evaluate the usefulness of the artificial substrate sampler in collecting macroinvertebrates for water quality assessment of Polish lowland rivers. This paper presents the results of a comparative study between two different sampling techniques, i.e. nettings filled with brick as artificial substrates and handnet sampling. The validity of applying the ...

Habitat colonization is a fundamental process in marine population dinamics and community ecology. Marine invertebrate colonization of artificial hard substrates and its spatial variation was studied over the course of one year at La Azufrada coral reef, Gorgona island, Colombia. Five sets of artificial plates (terracotta and ceramic) were deployed parallel to the bottom in each of three reef z...

The enzyme maltase (glucoinvertase; glucosidosucrase; maltase-glucoamylase; a-glucopyranosidase; glucosidoinvertase; a-D-glucosidase; a-glucoside hydrolase; a-1,4-glucosidase EC 3.2.1.20), is involved in the exo-hydrolysis of 1,4-a-glucosidic linkages and certain oligosaccharides into glucose which is an important energy source for soil microbes. This enzyme originates from different sources, w...