Trypsin from the pyloric caeca of Pacific cod (Gadus macrocephalus) was easily prepared by affinity chromatography on Benzamidine Sepharose 6B and gel filtration on Superdex 75. Pacific cod trypsin was composed of three isozymes, and their molecular masses were estimated 23,756.34 Da, 23,939.62 Da, and 24,114.81 Da by desorption/ionization time-of-flight mass spectroscopy (MALDI/TOF-MS) and the...

The ultrasound-assisted (UA) soybean oil methanolysis using KOH as a catalyst was studied at different reaction conditions in a microreactor. Box–Behnken experimental design, with three variables, was performed and the effects of three reaction variables i.e. reaction temperature, catalyst concentration and the methanol-to-oil molar ratio on fatty acid methyl ester (FAME) yield were evaluated b...

We determined the effects of mono-L-arginine-containing compounds on pial arterioles of anesthetized piglets. A closed cranial window was implanted, and the diameter of one pial arteriole was determined by intravital microscopy. Diameter was determined during application of artificial cerebrospinal fluid containing no drugs and during application of 10-5 10-4, 10-3, and 10-2 M L-arginine (ARG),...

The formation of liver metastases involves interactions between intravascular cancer cells and the hepatic microvasculature. Here we provide evidence that the arrest of intravascular B16F1 melanoma cells in the liver induces a rapid local release of nitric oxide (NO) that causes apoptosis of the melanoma cells and inhibits their subsequent development into hepatic metastases. B16F1 melanoma cel...

A series of 14 4-nitroanilide substrates and 17 thioester substrates have been used to measure kinetic constants with porcine pancreatic kallikrein. All of the substrates have a P1 arginine residue. The 4-nitroanilide substrates consist of seven P2-glycine and seven P2-phenylalanine tripeptides. As expected from previous results, the phenylalanine series substrates were generally 100-fold 'bett...

1. The present study examined effects of arginine (Arg) and various Arg analogues on the vascular tone of rabbit and rat aortic rings, the release of nitrite from cultured bovine aortic endothelial cells and the metabolism of L-Arg in bovine and porcine endothelial cell homogenates. The respective D-enantiomers or N-alpha-benzoyl-L-arginine ethyl ester did not substitute for L-Arg. 2. In bovine...

We examined the cytoprotective effect of L-arginine on gastric damage induced by 0.6 N HCl in rats and investigated whether the mechanism of this action is related to the nitric oxide (NO)-mediated protection. The animals were given 0.6 N HCl by gavage and killed 1 hr later. L-Arginine (100, 300 and 750 mg/kg) given p.o. 30 min before HCl treatment prevented these lesions in a dose-dependent ma...

Abstract Arsenic (As) negatively affects plant development. This study evaluates how the application of silicon (Si) can favor formation adventitious roots in rice under arsenate stress (AsV) as a mechanism to mitigate its negative effects. The simultaneous AsV and Si up-regulated expression genes involved nitric oxide (NO) metabolism, cell cycle progression, auxin (IAA, indole-3-acetic acid) b...

High blood pressure is an important health problem because of its prevalence and several complications. The high cost management the side effects conventional drugs have led patients to use medicinal plants. Carissa edulis one plants used in traditional hypertension. aim this study was determine crude extract fractions L-NAME hypertension rats models. phytochemical analysis extracts carried out...

BACKGROUND Vascular endothelial growth factor (VEGF), an endothelial cell mitogen that promotes angiogenesis, was initially identified as a vascular permeability factor (VPF). Abundant evidence suggests that angiogenesis is preceded and/or accompanied by enhanced microvascular permeability. The mechanism by which VEGF/VPF increases vascular permeability (VP), however, has remained enigmatic. Ac...