The endoplasmic reticulum (ER) comprises an interconnected membrane network, which is made up of lipid bilayer and associated proteins. This organelle plays a central role in the protein synthesis and sorting. In addition, it represents the synthetic machinery of phospholipids, the major constituents of the biological membrane. In this process, phosphatidic acid (PA) serves as a precursor of al...

We have studied the capacity of human neutrophils to release arachidonic acid from diacylglycerol, employing 1-stearoyl-2-[1-14C]arachidonoyl-sn-glycerol and 1-[1-14C]stearoyl-2-arachidonoyl-sn-glycerol as exogenous substrates. We have found that arachidonic acid is removed from diacylglycerol by the sequential action of two enzymes. First, the sn-1 position is split by 1-diacylglycerol lipase ...

We previously described 3 bioactive oxidation products of 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (PAPC) containing oxovaleroyl (POVPC), glutaroyl (PGPC), and epoxyisoprostane (PEIPC) groups at the sn-2 position that were increased in minimally modified/oxidized low density lipoprotein (MM-LDL) and rabbit atherosclerotic lesions. We demonstrated specific and contrasting effect...

In the present study, interactions of dopamine receptor agonists and antagonists with water swimming stress (WSS) on naloxone-induced jumping in morphine-dependent mice were examined. Mice were rendered dependent as described in the methods section. The opioid receptor antagonist, naloxone (1 mg/kg), was injected to elicit jumping (as a withdrawal sign). The first group exposed to WSS in the pr...

cofe2o4 magnetic nanoparticles were modified by dopamine (da) and ascorbic acid (aa) as anchors. separation of the da and aa using cofe2o4 magnetic nanoparticles have been studied by investigating the effects of ph, concentration of the da and aa, amount of adsorbents, contact time, ionic strength and temperature. the mechanism of adsorption was also studied. the adsorption of da and aa to the ...

objective(s) the aim of this study was to evaluate the effects of dexamethasone on striatal dopaminergic, glutamatergic and gamma amino butyric acid (gaba) ergic neurotransmission in normal and parkinsonian rats. materials and methods dexamethasone (0.15, 0.30, 0.60 and 0.8 mg/kg) was administered to normal or parkinsonian rats (i.p.) followed by the analysis of the striatal neurotransmitters c...

the purpose of the present study was to determine the role of medial prefrontal cortex (mpfc) dopaminergic system in fear conditioning response considering individual differences. animals were initially counterbalanced and classified based on open field test, and then were given a single infusion of the dopamine agonist, amphetamine (amph) and antagonist, clozapine (clz) into the medial prefron...

Dopamine (1) is a key neurotransmitter whose impact on pharmacological processes mediated by family of dopamine receptors designated D1, D2, D3, D4, and D5. Various diseases conditions such as schizophrenia, drug abuse, depression, restless leg syndrome, Parkinson’s disease (PD), inflammatory have been linked to aberrant D3 activity. Herein, we report series novel ligands with improved solubili...

Abstract Two sensitive and effective methods were developed for the detection of catecholamines related biogenic amines (dopamine, epinephrine, norepinephrine, serotonin, levodopa tyramine) using high performance liquid chromatography with fluorescence capillary electrophoresis laser-induced detection. A BODIPY fluorescent dye, 1, 3, 5, 7-tetramethyl-8-( N -hydroxysuccinimidyl propionic ester)-...