The development of therapeutic strategies that overcome the unique problems posed by Duchenne muscular dystrophy (DMD) has lead to the development of many contemporary approaches to human disease in general. Various treatment approaches have been explored--such as pharmacological therapies and cell-based, cytokine, and genetic therapies--that are all targeted to specific features of dystrophic ...

Replication of the IncL/M plasmid pMU604 is controlled by a small antisense RNA molecule (RNAI), which, by inhibiting the formation of an RNA pseudoknot, regulates translation of the replication initiator protein, RepA. Efficient translation of the repA mRNA was shown to require the translation and correct termination of the leader peptide, RepB, and the formation of the pseudoknot. Although th...

Antisense RNA probes were synthesized by in vitro transcription incubating 1 μg linearized plasmid, 2 μL 100 mM dithiothreitol (DTT), 1.3 μL NTP mixture (15.4 mM each ATP, CTP, GTP, and 10 mM UTP), 0.7 μL 10 mM digoxigenin-UTP or fluorescein-UTP (Roche Applied Science, Indianapolis, IN, USA), and 0.5 μL RNAguardTM (Amersham Biosciences, Piscataway, NJ, USA), 2 μL 10× transcription buffer, 1 μL ...

1 Animal Scientist, D.Sc., Adjunct Professor, Department of Animal Science, Universidade Federal de Viçosa, ViçosaMG, 36571-000, CNPq and INCT-Ciência Animal researcher ([email protected]) 2 Animal Scientist, D.Sc., Titular Professor, Department of Animal Science, Universidade Federal de Viçosa, Viçosa-MG, Brazil, 36571-000, CNPq and INCT-Ciência Animal researcher ([email protected]) 3 Agronomy Engi...

With increasing knowledge on the molecular mechanisms of endogenous RNA interference, small interfering RNAs (siRNAs) have been emerging as innovative nucleic acid medicines for treatment of incurable diseases such as cancers. Although several siRNA candidates for the treatment of ocular and respiratory diseases are undergoing clinical trials, there are challenges inherent in the further develo...

We recently demonstrated that C-CAM, an epithelial-cell adhesion molecule of the immunoglobulin supergene family, could be regulated by androgen and might act as a growth repressor during differentiation of the prostatic epithelium. To define the role of C-CAM in prostatic tumorigenesis, a tumorigenic human prostatic cancer cell line, PC-3, was transfected with an expression plasmid containing ...

The par stability determinant of Enterococcus faecalis plasmid pAD1 is the only antisense RNA-regulated addiction module identified to date in gram-positive bacteria. par encodes two small, convergently transcribed RNAs, designated RNA I and RNA II, that function as the toxin (Fst)-encoding and antitoxin components, respectively. Previous work showed that structures at the 5' end of RNA I are i...

The preS2 domain is the minimal functional unit of transcription activators that is encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC). To further understand the functional role of PreS2 in hepatocytes, a PreS2 expression plasmid, pcS2, was constructed and stably transfected into HepG2 cells. W...