BACKGROUND Platelet depletion is a key feature of hemolytic uremic syndrome (HUS) caused by Shiga toxin-producing Escherichia coli (STEC) infection. The mechanism underlying STEC-induced platelet depletion, however, is not completely understood. METHODOLOGY/PRINCIPAL FINDINGS Here we demonstrated for the first time that platelet surface expression of CD47 was significantly decreased in C57BL6...

We report the immunochemical characterization of a new platelet-specific alloantigen detected using an lgG antibody isolated from the serum of a patient with posttransfusion purpura (PTP). In indirect immunoprecipitation experiments, the antibody, termed anti Leka, predominantly precipitated glycoprotein (GP) llb from Triton X-100 lysates of normal human platelets. In an immunoblot procedure, w...

We report the immunochemical characterization of a new platelet-specific alloantigen detected using an lgG antibody isolated from the serum of a patient with posttransfusion purpura (PTP). In indirect immunoprecipitation experiments, the antibody, termed anti Leka, predominantly precipitated glycoprotein (GP) llb from Triton X-100 lysates of normal human platelets. In an immunoblot procedure, w...

background: platelets rapidly lose their qualities usually after 5 day of storage. different standard methods have been recommended to check the quality of platelets during storage which some of them show better correlation with other quality markers during storage. the purpose of this study was to demonstrate if platelet factor 3 (pf3) assay could be an indicator of storage lesion and provide ...

The analysis of sera containing different platelet-reactive antibodies, eg, autoantibodies, platelet-specific alloantibodies like anti-PIA1, -PIA2, -Baka, and HLA antibodies, is still difficult. Recently, monoclonal antibodies against major platelet membrane constituents (glycoproteins IIb/IIIa and Ib and HLA class I molecule) have become available. In this report we describe a new assay that t...

We report the immunochemical characterization of a new platelet-specific alloantigen detected using an lgG antibody isolated from the serum of a patient with posttransfusion purpura (PTP). In indirect immunoprecipitation experiments, the antibody, termed anti Leka, predominantly precipitated glycoprotein (GP) llb from Triton X-100 lysates of normal human platelets. In an immunoblot procedure, w...

Thromb Haemost 2009; 101: 217–219 Anti-platelet agents are a cornerstone in the treatment of symptomatic coronary artery disease and acute coronary syndromes. Despite the proven benefits of acetylsalicylic acid (ASA) and clopidogrel, ischaemic events still occur in patients receiving dual anti-platelet therapy. Over the course of the last 10 years, a series of published studies has examined the...

As of today, no practical method for large-scale functional anti-thrombosis agent screening exists. Based on the phenomenon that platelet activation results in the release of ATP from dense granules, we report the development and optimization of a 96-well microplate luciferase assay to assess platelet activation via luminescence detection of the released ATP. In addition, the assessment of re-c...

conclusions the diagnosis of thrombocytosis is not easy in all cases. the coexistence of anca and the immune complex in cgn may cause a severe inflammatory state, leading to extreme rt. the roles that the immune complex and anca play on the effect of the platelet count and function in cgn need further research. introduction we describe a female patient with extreme reactive thrombocytosis (rt) ...

Staphylococcus aureus can stimulate activation and aggregation of platelets, which are thought to be factors in the development of infective endocarditis. Previous studies have identified clumping factor A (ClfA) and fibronectin binding proteins A and B (FnBPA and FnBPB) as potent platelet aggregators. These proteins are able to stimulate rapid platelet aggregation by either a fibrinogen- or a ...