To gain insight into the cell envelope of Escherichia coli grown under aerobic and anaerobic conditions, lipoproteins were examined by using functional genomics. The mRNA expression levels of each of these genes under three growth conditions--aerobic, anaerobic, and anaerobic with nitrate--were examined by using both Affymetrix GeneChip E. coli antisense genome arrays and real-time PCR (RT-PCR)...

The fluorescent compound trisodium, 3-hydroxy-5,8,10-pyrenetrisulfonate (PTS) was used to quantify the apoplastic flux through red pine (Pinus resinosa Ait.) root systems-that portion of the total water flux reaching the xylem without ever crossing a semipermeable membrane. Flow was induced by pressure through detopped root systems, and by transpiration through intact seedlings. Apoplastic flux...

(1) Potential uses of photosynthetie bacteria in the recycling of human wastes have been considered. I t is concluded that systems using photosynthetic bacteria may have advantages over purely algal systems under conditions where total recycling is needed and energy is not abundant. (2) Two systems are proposed in which the metabolic potential of the photosynthetic bacteria might be realized. B...

(1) Potential uses of photosynthetie bacteria in the recycling of human wastes have been considered. I t is concluded that systems using photosynthetic bacteria may have advantages over purely algal systems under conditions where total recycling is needed and energy is not abundant. (2) Two systems are proposed in which the metabolic potential of the photosynthetic bacteria might be realized. B...

(1) Potential uses of photosynthetie bacteria in the recycling of human wastes have been considered. I t is concluded that systems using photosynthetic bacteria may have advantages over purely algal systems under conditions where total recycling is needed and energy is not abundant. (2) Two systems are proposed in which the metabolic potential of the photosynthetic bacteria might be realized. B...

Anaerobic conditions were employed to study the relationship between membrane potential and chemotaxis in Spirochaeta aurantia. When cells were grown anaerobically and suspended in anaerobic potassium phosphate buffer (pH 5.5), membranes did not appear to be polarized. Nevertheless, motility was supported by a transmembrane pH gradient, and the anaerobic cells exhibited D-xylose taxis. Introduc...

Nitrate reductase activity in barley (Hordeum vulgare L. cv. Himalaya) aleurone layers has been determined in the intact tissue, using two different methods. The first method measures the rate of appearance of H(2) (18)O produced during the reduction of KN(18)O(3). The second assay measures excreted nitrite resulting from nitrate reduction under anaerobic conditions. Nitrite production in this ...

Methanogenic flowthrough aquifer columns were used to investigate the potential of bioaugmentation to enhance anaerobic benzene-toluene-ethylbenzene-xylene (BTEX) degradation in groundwater contaminated with ethanol-blended gasoline. Two different methanogenic consortia (enriched with benzene or toluene and o-xylene) were used as inocula. Toluene was the only hydrocarbon degraded within 3 years...

Candida guilliermondii was isolated from sterile specimens with increasing frequency over a several-month period despite a paucity of clinical evidence suggesting true Candida infections. However, a health care-associated outbreak was strongly considered due to growth patterns in the microbiology laboratory that were more consistent with true infection than environmental contamination. Therefor...

As a result of the studies of Locke, Main, and Mellon (1938), Fox, German, and Janeway (1939), and others, and the chemical experiments of Shaffer (1939), all of whom have advocated the importance of the presence of oxygen and the formation of hydrogen peroxide as essential to the action of sulfanilamide, interest has developed as to whether sulfanilamide can exert its action on organisms in vi...