Arginine residues in barley a-amylase/subtilisin inhibitor (BASI) involved in binding to barley a-amylase 2 (AMY2) were differentially labelled using AMY2 as protectant and phenylglyoxal (PGO) and [14C]PGO as modifying agents. Chymotryptic fragments of labelled BASI were purified by reverse-phase HPLC, and we concluded that the radiolabelled Arg-27, Arg-155 and most likely Arg-127, identified b...

The structure-based design, synthesis, and screening of a glucuronic acid scaffold library of affinity ligands directed toward the catalytic cleft on porcine pancreas alpha-amylase are presented. The design was based on the simulated docking to the enzyme active site of 53 aryl glycosides from the Available Chemicals Directory (ACD) selected by in silico screening. Twenty-three compounds were s...

In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the α-globulin, the 33 kDa glyoxalase I (Gly I), the 52-63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of sev...

We evaluated a new kinetic assay for alpha-amylase (Phadebas IsoAmylase Test), using modified starch as the substrate and a CentrifiChem 400 centrifugal analyzer system. We determined isoamylase activities by using a selective inhibitor. Results were compared with those obtained with the chromogenic Phadebas dyed-starch technique. The method for total amylase appeared to be rapid and precise (C...

The management of postprandial hyperglycemia is an important strategy in the control of diabetes mellitus and complications associated with the disease, especially in the diabetes type 2. Therefore, inhibitors of carbohydrate hydrolyzing enzymes can be useful in the treatment of diabetes and medicinal plants can offer an attractive strategy for the purpose. Vaccinium arctostaphylos leaves are c...

Bacterial amylase from Bacillus subtilis and fungal amylase from Aspergillus niger were potential source for industrial exploitation in the field of amylase production. Hence, this study on bacterial amylase and fungal amylase was done to compare the kinetic parameter of both the types of amylases. 30-60% ammonium sulfate precipitation has done which showed 90 % of purification of both the part...

Physalis minima Linn. (Family  Solanaceae) is commonly known as Ground Cherry or Sunberry. It is traditionally used as diuretic, purgative, analgesic, anthelmentic, febrifuge, vermifuge, abortificient, etc. Many steroidal lactones have been identified from the plant and it has been reported to possess antifertility, hypoglycemic, cytotoxic, antiulcer, antibacterial, anti-inflammatory, analgesi...

Cholecystokinin (CCK) has been shown to activate RhoA and Rac1, as well as reorganize the actin cytoskeleton and, thereby, modify acinar morphology and amylase secretion in mouse pancreatic acini. The aim of the present study was to determine which heterotrimeric G proteins activate RhoA and Rac1 upon CCK stimulation. Galpha(13), but not Galpha(12), was identified in mouse pancreatic acini by R...

Peanut (Arachis hypogea L.) industrial processing generates underused by-products, which are mostly discharged. In this work, extracts from peanut by-product were obtained by Supercritical Fluid Extraction (SFE), Pressurized Liquid (PLE) and Subcritical Water (SWE), compared to Soxhlet (SOX). Yield values varied 12.94% 37.65% for SOX-water SWE, respectively. The quality of the was evaluated tot...