The alpha-amylase (alpha-1,4-glucan 4-glucanohydrolase, EC 3.2.1.1) of Bacillus subtilis strain W23 is less negatively-charged than the alpha-amylase of B. amyloliquefaciens strain F, as determined by electrophoretic mobility in polyacrylamide gel at pH 8.6. The alpha-amylase of strain W23 is immunologically unrelated to the alpha-amylase of strain F, as judged by lack of cross-reaction in Ouch...

We report the characterization and cDNA cloning of two alpha-amylase isozymes from larvae of the Western corn rootworm (Diabrotica virgifera virgifera LeConte). Larvae raised on artificial media have very low levels of amylase activity, and much higher levels are found in larvae raised on maize seedlings. At pH 5.7, the optimum pH for enzyme activity, the alpha-amylases are substantially but no...

Sequencing upstream of the Streptococcus mutans gene for a CcpA gene homolog, regM, revealed an open reading frame, named amy, with homology to genes encoding a-amylases. The deduced amino acid sequence showed a strong similarity (60% amino acid identity) to the intracellular a-amylase of Streptococcus bovis and, in common with this enzyme, lacked a signal sequence. Amylase activity was found o...

This study established a novel non-invasive and efficient analysis method which can be applied in research of individual’s psychological stress. Because in the different state of psychological stress, the oral secretion of salivary α-amylase will be changed under the control of the hypothalamuspituitary-adrenal medulla secretion system. Many studies have confirmed that salivary α-amylase measur...

Besides the high starch content, sweet potato contains endogenous amylases where the two Predominants are - and β-amylases. Sweet potato is thought to be a promising source of β -amylase since β -amylase is one of the major protein in the tubers. This investigation has focused on the properties of β amylase from white-flesh sweet potato that has been grown in Iran as a potential source for indu...

Highlight: The level of pathogen alpha-amylase and plant beta-amylase activities could be components of plant-pathogen interaction associated with the resistance of triticale to Fusarium head blight. Triticale was used here as a model to recognize new components of molecular mechanism of resistance to Fusarium head blight (FHB) in cereals. Fusarium-damaged kernels (FDK) of two lines distinct in...

A study was conducted to evaluate the effects of dietary α-amylase and β-xylanase supplementation of corn-soy diets, formulated with or without supplemental phytase, on growth performance, energy utilization, and starch digestibility in broiler chickens. A total of 336 slow-feathering, Cobb × Cobb 500 male broilers were randomly distributed to 6 treatments having 8 replicates of 7 birds each. B...

Elevation of serum amylase level is usually associated with pancreatitis, but 2% of cases are associated with medication. Only 1 case of pancreatitis caused by sertraline has been reported. Although it has been reported that selective serotonin reuptake inhibitors may rarely cause pancreatitis, Elevated serum amylase levels associated with sertraline use in cases without pancreatitis has not be...

A pigeon pancreas microsomal system supplemented with microsomal supernatant fluid or pH 5 enzyme incorporates radioactive amino acids into purified amylase. Several parameters of the incorporation system are presented. TrypCc digestion of the isolated radioactive amylase shows that the amino acids are incorporated into peptide linkage within the protein, as in amylase synthesized in viva. The ...

Serum enzyme activity was sequentially determined in 10 consecutive patients with diabetic ketoacidosis, of whom all had increased -glucuronidase activity, eight had increased amylase activity, and four had increased acid phosphatase activity. Activity of amylase and that of the two lysosomal enzymes were poorly correlated, irrespective of whether peak activities or activities of all samples we...