A method for the determination of the DNA content of isolated nuclei of different ploidy has been developed. It is based on measurement of the nuclear dry mass, with an integrating microinterferometer, before and after DNase treatment. The values found are slightly low, because, as indicated by biochemical determinations, consistently 5% to 8% of DNA is not extracted by DNase under these condit...

Caffeine is one of the main substances inculpated in the production of soft drinks. A variety of compounds are also used to preserve soft drinks; benzoic acid happens to be one of the preservatives. Caffeine and benzoic acid were isolated from four selected soft drinks – Coca Cola, Pepsi Cola, Mirinda and Fanta cocktail – using methylene chloride. For caffeine, the average absorbance obtained w...

A nano-hybrid based on polystyrene derivatives and magnetite was introduced as a new sorbent for pretreatment and determine the trace amount of malachite green from aqueous solution by spectrophotometry after preconcentration by solid phase extraction (SPE). UV–Vis spectrophotometer was used for determination of MG concentration after desorption of the dye by hydrochloric acid solution in the s...

Background & Aim: Papaver bracteatum Lindl.  (Papaverace) is an important pharmaceutical plant which contains isoqinoline alkaloids. Isoqinoline alkaloids constitute one distinct group of secondary metabolites of the Papaverace family. Codeine and thebaine are important alkaloids of Papaver bracteatum which use widely due to its medicinal properties. Therefore it is...

The soil test analysis conducted by research on variety of crop growing soil gave values of 1220ppm of Phosphate which is regarded to be adequate for plant establishment and production. In the case of Sulphate, the experimental results obtained were as follows: virgin land (14.8ppm), tomato land (20.9ppm), cassava land (43.3ppm). Soil test analysis gave standard sulphate values required for gro...

A spectrophotometric procedure for the determination of ascorbic acid in pure form and in a number of pharmaceutical preparations and real samples has been developed that offers the advantages of simplicity, accuracy, precision and sensitivity over many other methods. The method is based on the oxidation of ascorbic acid by a known excess amount of potassium chromate followed by the estimation ...

Minced meats are offered to the consumers with different levels of fat. In addition, they are the main ingredients in a large assortment of processed products like hamburgers, patties and sausages. There is a need for a fast determination technique of different chemical composition in minced meat to ensure that the right amount is presented. In this study, a pushbroom hyperspectral imaging syst...