The hydrolysis of sodium borohydride as a source of hydrogen gas is studied at different mole ratios of H+ to NaBH4. The sulfonated porous carbon (SPC) is used as a source of hydrogen ion and catalyst. It is found that the sulfonated porous carbon exhibits high activity for the hydrolysis of NaBH4 to generate hydrogen gas at room temperature in comparison to Amberlyst-15 and Nafion-NR50. The ki...

BACKGROUND Polymorphic thiopurine S-methyltransferase (TPMT) is a major determinant of thiopurine toxicity. METHODS We extracted 6-thioguanine nucleotides (6-TGNs) and 6-methylmercaptopurine nucleotides (6-MMPNs) from erythrocytes with perchloric acid and converted them to 6-thioguanine (6-TG) and a 6-methylmercaptopurine (6-MMP) derivative during a 60-min acid hydrolysis step. The liquid chr...

The hydrolysis of sodium borohydride as a source of hydrogen gas is studied at different mole ratios of H+ to NaBH4. The sulfonated porous carbon (SPC) is used as a source of hydrogen ion and catalyst. It is found that the sulfonated porous carbon exhibits high activity for the hydrolysis of NaBH4 to generate hydrogen gas at room temperature in comparison to Amberlyst-15 and Nafion-NR50. The ki...

Hydrolysis of organic sulfate esters proceeds by two distinct mechanisms, water attacking at either sulfur (S-O bond cleavage) or carbon (C-O bond cleavage). In primary and secondary alkyl sulfates, attack at carbon is favored, whereas in aromatic sulfates and sulfated sugars, attack at sulfur is preferred. This mechanistic distinction is mirrored in the classification of enzymes that catalyze ...

a-N-Acetyl-D-galactosaminidase (EC 3.2.1.49) was purified from porcine liver by acid and ammonium sulfate precipitation, followed by successive chromatography on DEAE-cellulose, concanavalin A-Sepharose, DEAEcellulose with ampholyte elution, Sephadex G-150, and hydroxylapatite. The p-nitrophenyl-a-N-acetylgalactosaminide and Forssman hapten hydrolyzing activities were purified 3,300-fold and 19...

The use of the gaseous funaigant-nematicide methyl bromide in agriculture is scheduled to be phased out in the year 2001.1,3-Dichloropropene (1,3-D) in combination with chloropicrin and an herbicide is considered to be a viable alternative to methyl bromide for some crops. 1,3-Dichloropropene consists of two isomers, cis- and trans-l,3-D. A number of soil bacteria have been shown to initially d...

One of the fundamental attributes a liquid chromatography column is its stability when exposed to acidic and basic mobile phases. However, there have been relatively few reports date on hydrophilic interaction (HILIC) columns. Here, we report results evaluations carried out for HILIC columns packed with ethylene-bridged hybrid or silica particles using accelerated conditions, employing 100% aqu...

Many environmental contaminants are amenable to degradation by alkaline hydrolysis. This project explored the use of ammonia gas to raise soil pH in order to stimulate alkaline hydrolysis. When ammonia gas dissolves in water, it forms ammonium ion, which consumes hydrogen ions (H+), thereby increasing pH. This study established that 5% ammonia in air can increase soil pH from 7.5 to 10.3. Batch...