Corn (Zea mays L.) root tonoplast vesicles were treated with the primary-amine specific reagent, fluorescamine (FL). Modification by FL caused a differential inhibition to the coupled activities of tonoplast H(+)-ATPase. Within the range of 0 to 5 micromoles of FL per milligram of protein, the proton pumping rate was significantly reduced but ATP hydrolysis was only slightly affected. Yet, the ...

A quartz crystal microbalance (QCM) biosensor based on polydopamine (PDA) surface was developed for real-time analysis of the binding kinetics of protein-protein interactions. The biosensor was fabricated by simply immersing the gold sensor chip into an aqueous dopamine solution at pH 8.5 leading to a spontaneous deposition of PDA film onto the sensor chip surface, which was followed by incubat...

Microfibril-associated glycoprotein-1 (MAGP-1) is a small molecular weight protein associated with extracellular matrix microfibrils. Biochemical studies have shown that MAGP-1 undergoes several posttranslational modifications that may influence its associations with other microfibrillar components. To identify the sites in the molecule where posttranslational modifications occur, we expressed ...

A modification of the GLEAMS model was used to determine application windows which would optimise efficacy and environmental safety for herbicide application to a forest site. Herbicide/soil partition coefticients were determined using soil samples collected from the study site for two herbicides (imazapyr, K,=46, triclopyr ester, K,,=l038)andpublishedvaluesfortwootherherbicides(henarinone,K,=5...

removal of typical contaminants like aromatic amines (e.g. 1-naphthalen amine, diphenyl amine, aniline, 2 and 6-dimethyl pyridine) from water during one-stage process is a method to purify water wherein organic contaminants get extracted from water by bentonite. in this study the effects of ph, stirring time, stirring speed, bentonite mass and bentonite size (mesh) were investigated. also, the ...

the solubility of co2 in the primary, secondary, tertiary and sterically hindered amine aqueous solutions at various conditions was studied. in the present work, the modified kent-eisenberg (m-ke), the extended debye-hückel (e-dh) and the pitzer models were employed to study the solubility of co2 in amine aqueous solutions. two explicit equations are presented to evaluate the concentration of h...

Site-specific covalent modification of RNA is important for enabling structure–function studies. For example, probes such as fluorescein are commonly used in fluorescence resonant energy transfer (FRET) investigations of RNA folding. Biotin is used for immobilization during single-molecule analysis, to enable RNA–protein cross-linking studies, and as a key element of selection schemes in vitro....

High surface area alumina coatings were prepared on surface acoustic wave (SAW) mass balances. These coatings were fabricated by anodic etching of evaporated aluminum films. The coatings consisted of roughly collinear pores penetrating through the monolithic alumina film. The nanoporous (NP) coatings were characterized by scanning electron microscopy, and the pore number density and diameter we...

Tumors frequently contain hypoxic regions that result from a shortage of oxygen due to poorly organized tumor vasculature. Cancer cells in these areas are resistant to radiation- and chemotherapy, limiting the treatment efficacy. Macrophages have inherent hypoxia-targeting ability and hold great advantages for targeted delivery of anticancer therapeutics to cancer cells in hypoxic areas. Howeve...