The optical response of an alkaline phosphatase (ALP) probe was evaluated against various bacterial species.

objectives: hyperlipidemia can cause a variety of diseases such as atherosclerosis, diabetes, and fatty liver which is followed by increased liver enzymes. since berberis vulgaris (b. vulgaris) root possesses antioxidant properties, the present study was conducted to investigate the effect ofits extract on the activity of liver enzymes in rats. materials and methods: in this experimental study,...

Lansoprazole, a known H(+)/K(+)-ATPase inhibitor, is currently used as a therapeutical option for the initial treatment of gastroesophageal reflux disease. Recently, lansoprazole has been found to be an inhibitor of cytosolic PHOSPHO1 (a phosphatase which hydrolyses phosphocholine and phosphoethanolamine), providing a possible therapeutical target to cure pathological mineralization. Since PHOS...

A technique for measuring the alkaline phosphatase activity of serum protein fractions separated by electrophoresis on cellulose acetate strips is described. Alkaline phosphatase activity in alpha 2, alpha 1, and beta globulins and in albumin is present in varying proportions in normal and pathological sera. In normal sera peak activity is in the alpha 2 globulins and beta globulins have a litt...

protein-X in cholestasis. Clin Chim Acta 67, 269-279 (1976). 7. Crofton PM, Smith AF. Alkaline phosphatase of high and low molecular mass in human serum and bile: A comparative study of kinetic properties. Clin Chem 26, 451-456 (1980). 8. Plomteux G, Reginster N. Mesure des fractions hepatique, intestinale et osseuse des phosphatases alcalines du serum. Ann Biol Clin 38, 215-222 (1980). 9. Lean...

We have constructed a vector designed to facilitate the study of protein secretion in Bacillus subtilis. This vector is based on a translational fusion between the expression elements and signal sequence of Bacillus amyloliquefaciens alkaline protease and the mature coding sequence for Escherichia coli alkaline phosphatase (phoA). We show that export of alkaline phosphatase from B. subtilis dep...

The present study was designed to identify alkaline phosphatases in non-permeabilized hyphal cells of the fungus Neurospora crassa by staining these enzymatic activities with a modified azo dye coupling method. Our strategy allowed the identification of three non-specific alkaline phosphatase activities, one of them possibly being a novel putative enzyme, which is not responsive to either Mg(2+...