* For DPN synthesis, 1.0 ml. of reaction mixture contained 50 y of the enzyme preparation, 0.3 micromole of MgCl 2, and 50 micromoles of glycylglycine buffer (pH 7.4) in addition to ATP and NMN; for ATP synthesis 1.0 ml. contained 50 y of the enzyme preparation, 0.75 micromole,of MgC12, and 50 micromoles of glycylglycine buffer (pH 7.4) in addition to DPN and P-P. Constant values were reached a...

Serine hydroxymethylase catalyzes the reversible cleavage of several P-hydroxy-a-amino acids to glycine and the appropriate aldehyde. With D-alanine as substrate, the a-hydrogen is labilized and a slow transamination to pyruvate and the pyridoxamine phosphate enzyme occurs. The absolute stereochemistry of this transamination has been determined by using pyridoxamine pyruvate transaminase to ana...

Saline–alkali stress is an important environmental factor affecting the growth and development of plants. Plants affected by saline–alkali can mitigate damage regulating content osmoregulatory substances such as soluble sugars. Elucidating regulatory mechanism changes in sugar fractions jujube fruits under crucial for fruit industry saline areas. In this study, we investigated effects on conten...

AIM To evaluate the therapeutic effect of Nodinhibit-1 on alkali-burn-induced corneal neovascularization (CNV) and inflammation. The nucleotide-binding oligomerzation domain 1 (NOD1) is a potent angiogenic gene. METHODS The alkali-burned rat corneas (32 right eyes) were treated with eye drops containing Nodinhibit-1 or phosphate buffered solution (PBS, PH 7.4) only, four times per day. CNV an...

The large scale, respiration-linked accumulation of Ca+f ions by isolated mitochondria (l-lo), which leads to deposition of amorphous granules of calcium phosphate (5, 7, 8, lo-12), has been found to be accompanied by accumulation of significant amounts of a phosphate derivative (or derivatives) hydrolyzed to inorganic phosphate by boiling in 1 N HCl for 7 minutes (5). In this paper it is demon...

Potassium ions at low concentrations stimulate cytokinin-dependent betacyanin synthesis in Amaranthus tricolor seedlings more than other alkali metal ions when tested as the chloride salts. The sequence of relative stimulation is K(+) > Rb(+) > (Na(+) = Li(+)). Calcium and Mg(2+) ions are inhibitory at concentrations > 1 millimolar when tested as chlorides. Anions also have an effect on the deg...

In the presence of nerve growth factor (NGF), PC12 pheochromocytoma cells undergo neuronal differentiation with a concomitant 3- to 5-fold increase in the specific level of an Mr = 230,000 cell surface component named the NGF-inducible large external, or NILE, glycoprotein. Antisera raised against NILE glycoprotein (NILE GP) purified from PC12 cells have been found to recognize most, if not all...

The cells of Saccharomyces cerevisiae accumulate inorganic polyphosphate (polyP) when reinoculated on a phosphate-containing medium after phosphorus starvation. Total polyP accumulation was similar at cultivation on both glucose and ethanol. Five separate fractions of polyP: acid-soluble fraction polyP1, salt-soluble fraction polyP2, weakly alkali-soluble fraction polyP3, alkali-soluble fractio...