The ability of human mesenchymal stem cells (hMSC) to differentiate into osteoblasts was examined through the use of osteogenic induction medium (MSCOIM) cultures. hMSC first attached to the dish surface and exhibited fibroblast-like spindle shapes, and after proliferation, formed cuboidal shapes. Calcium assays and the use of von Kossa and alizarin red S staining showed that hMSC were capable ...

A Sensitive and precise differentialpulse stripping voltammetric method at static mercury dropping electrode (SMDE) has been applied to measure zinc(II) by anodic stripping at pH∼4 and aluminum(III) by adsorptive cathodic stripping mode in presence of 1×10M N,N` -bis-2-hydroxyethyl-2-amino-ethane sulphonic acid (BES) buffer at pH~7.1 and 2×10 M of alizarin red S as a ligand in drinking water sa...

The dihydropyridine-type calcium channel blocker, benidipine (BD) has been widely used in hypertension therapy. Previous studies have demonstrated that BD has a positive effect on bone metabolism. Inspired by this promoting phenomenon, the present study investigated the effects of BD on osteoblasts in vitro. Experiments were designed and performed, including an MTT assay, reverse transcription-...

The Wnt/β-catenin pathway has a role in osteoblast differentiation and bone formation. We screened 100 plant extracts and identified an extract from Euodia sutchuenensis Dode (ESD) leaf and young branch as an effective activator of the Wnt/β-catenin pathway. ESD extract increased β-catenin levels and β-catenin nuclear accumulation in murine primary osteoblasts. The ESD extract also increased mR...

The virulence of a urease-negative mutant of uropathogenic Proteus mirabilis and its wild-type parent strain was assessed by using a CBA mouse model of catheterized urinary tract infection. Overall, catheterized mice were significantly more susceptible than uncatheterized mice to infection by wild-type P. mirabilis. At a high inoculum, the urease-negative mutant successfully colonized bladders ...

Pycnogenol (PYC) is a natural plant extract from the bark of Pinus pinaster Atiton that has potent anti-oxidant activities. Recent studies have shown that orally administered PYC can increase the biomechanical strength of bone and bone mineral density in ovariectomized rats. However, the effect of PYC on osteoblast mineralization has not yet been examined. We studied the differentiation and min...

The biotechnology to immobilize biomolecules on material surfaces has been developed vigorously due to its high potentials in medical applications. In this study, a simple and effective method was designed to immobilize biomolecules via amine-N-hydroxysuccinimide (NHS) ester conjugation reaction using functionalized poly-p-xylylene coating on material surfaces. The NHS ester functionalized coat...

conclusions based on our findings, we conclude that the separation and reproduction of adipose tissue cells is an appropriate method for purification of mscs in animal studies. regarding the histomorphometric and flow cytometry analysis results, we demonstrated the differentiation ability of mscs in normal medium and hope to employ these cells for the regeneration of damaged bone tissues in the...

The fabrication of metal oxide nanofibers using (titanium (IV) isopropoxide) and (tin tert-butoxide) weight ratio 1:1 precursor in presence poly (vinyl pyrrolidone) as a binder well-known electrospinning technique is reported. average diameter TiO2, SnO2, composite TiO2-SnO2 were found to be the range 75–110 nm. characterized thermogravimetric analysis (TGA) understand polymer evaporation tempe...