The aim of this study was to further broaden the heterologous expression alginate lyase from Vibrio alginolyticus in a Bacillus subtilis vector. A B. WB600/pP43NMK-alg62 strain constructed. (NH4)2SO4 precipitation and Ni-affinity chromatography were performed purify enzyme. We then characterized Its molecular weight 57.64 kDa, it worked optimally at 30 °C with pH 8.0. Ca2+ markedly enhanced enz...

The comparative study of food fibers preparations (“Vitacel” – wheat fiber, beet fibers, calcium alginate, TIPRO 601 – native collagen protein, pork rin d) in a chronic biological experiment has shown that none of them had cumulative toxic effect, and possessed a low level of food sensibilization. Calcium alginate, native collagen protein and pork rind help to increase calcium content in bones ...

Four hydrophilic polymers in the form of beads - chitosan, alginate, alginate/polyvinyl alcohol (PVA), and chitosan-coated alginate - were used as supports for lipase immobilisation. Hydrogel beads were characterised by bead-size-distribution estimation, surface morphology studies, and polymer interactions assessment. Matrix performances - loading efficiency, immobilisation yield, enzyme activi...

We report here the purification and characterization of phosphomannose isomerase-guanosine 5”diphospho-D-mannose pyrophosphorylase, a bifunctional enzyme (PMI-GMP) which catalyzes both the phosphomannose isomerase (PMI) and guanosine 5’-diphosphoD-mannOSe pyrophosphorylase (GMP) reactions of the Pseudomonas aeruginosa alginate biosynthetic pathway. The PMI and GMP activities co-eluted in the ...

We describe a method for creating alginate hydrogels with adjustable degradation rates that can be used as scaffolds for stem cells. Alginate hydrogels have been widely tested as three-dimensional constructs for cell culture, cell carriers for implantation, and in tissue regeneration applications; however, alginate hydrogel implants can take months to disappear from implantation sites because m...

The waste water and industrial effluent-treatment requires both inorganic and organic flocculants. Among the organic type, polymeric flocculants (synthetic as well as natural) are preferred because of their low dosage, easy handling, inertness to pH changes, production of large cohesive flocs and versatile tailorability. The concern for environmental and ecological issues warrants the use of th...

There is a requirement for the development of oral dosage forms that are adhesive and allow extended oesophageal residence time for localised therapies, or are non-adhesive for ease of swallowing. This study provides an initial assessment of the in vitro oesophageal retention characteristics of several widely utilised pharmaceutical coating materials. To this end, a previously described apparat...

Calcium alginate membrane has great potential for membrane separation technology. The polymer frameworks of the membrane were successfully regulated by the mass fraction of homopolymeric blocks of -L-guluronic acid (FGG) in the entire molecular chain of alginate and the additive CaCl2 as a cross-linker. The mechanical strength can be controlled by regulated the FGG and CaCl2 concentration. Sele...