Enzymatic hydrolysate of African yam bean seed protein isolate was prepared by treatment with alcalase. The hydrolysate was further fractionated into peptide sizes of <1, 1-3, 3-5 and 5-10 kDa using membrane ultrafiltration. The protein hydrolysate (APH) and its membrane ultrafiltration fractions were assayed for in vitro antioxidant activities. The <1 kDa peptides exhibited significantly bette...

Seal meat is of high nutritive value but currently a by-product and waste from seal oil processing. hydrolysates were generated alcalase catalysis subjected to soft dynamic digestion system study their nutritional the health benefits. The total amino acid content was determined as 568.54 384.65 mg/g for its intestinal digests, respectively, using UPLC, both samples rich in essential acids such ...

A pea protein isolate was hydrolyzed by a double enzyme treatment method in order to obtain short peptide sequences used as raw materials to produce lipopeptides-based surfactants. Pea protein hydrolysates were prepared using the combination of Alcalase and Flavourzyme. The influence of the process variables was studied to optimize the proteolytic degradation to high degrees of hydrolysis. The ...

In the present study, we investigated to the antioxidant and angiotensin I-converting enzyme (ACE) inhibitory activities of the northern shrimp (Pandalus borealis) by-products (PBB) hydrolysates prepared by enzymatic hydrolysis. The antioxidant and ACE inhibitory activities of five enzymatic hydrolysates (alcalase, protamex, flavourzyme, papain, and trypsin) of PBB were evaluated by the 2, 2′-a...

Enzymatic modification of proteins is being increasingly studied, with the aim of adding commercial and nutritional values to byproducts. Thus this work aims to study the hydrolysis degree obtained by different enzymes and compare among different Withemouth croaker (Micropogonias furnieri) byproducts: mechanically separated meat, protein isolate and bones. To obtain the peptides, an enzymatic h...

BACKGROUND β-lactoglobulin hydrolysates (BLGH) have shown antioxidant, antihypertensive, antimicrobial, and opioid activity. In the current study, an innovative combination of high hydrostatic pressure and enzymatic hydrolysis (HHP-EH) was used to increase the yield of short bioactive peptides, and evaluate the anti-inflammatory and antioxidant properties of the BLGH produced by the HHP-EH proc...

This work was aimed to study the preparation and hydrolyzation of cartilage from duck trachea to obtain crude chondrotin sulfate(CS). Duck trachea is comprised of 66.19 ± 0.96% protein and 3.46 ± 0.15% CS (dry basis). HPLC chromatograms revealed that CS of duck trachea contained higher content of ΔDi-4S than ΔDi-6S with trace amount of ΔDi-diSs. To obtain cartilage part of the trachea, use of a...

Abstract The most prevalent by-product produced by the brewery factory is brewer's spent grain (BSG). A total of 70%, 10%, and 20% BSG are used for animal feed, biogas production, landfills, respectively. Feeding wet can avoid cost drying. Wet as a replacement forage in diets animals. high moisture content ease deterioration leftover fresh feed drawbacks (3–5 days). provided low-cost despite it...

Whey protein (WP) has nutritional value, but the presence of β-lactoglobulin (β-LG) and α-lactalbumin (α-LA) cause allergic reactions. In this study, hypoallergenic whey hydrolyate (HWPH) was prepared by decomposing β-LG α-LA WP using exo- endo-type proteases. The enzyme mixing ratio reaction conditions were optimized response surface methodology (RSM). Degradation confirmed through gel electro...