Aminopeptidase C was purified from fresh chicken skeletal muscle by arnmonium sulfate fract;onation,andbysuccessivechromatographyonDEAE-cellulose,UltrogelAcA34,DEAE-cellulose again, and an alanine AH-Sepharose 4B ftMnity column twice. The purificd enzyme migrated as a s;ngle band by SDS-PAGE. Am;nopeptidase C was purified about 300-fo1cl over the crllde extraet with a },ield of O.6%. The molecu...

The major aminopeptidase from human quadriceps muscle was purified (as judged by polyacrylamide-gel electrophoresis) by anion-exchange chromatography (two steps) and gel filtration (two steps). The enzyme showed maximum activity at pH 7.3, in the presence of 1 mM-2-mercaptoethanol and 0.5 mM-Ca2+ ions; activation of the enzyme occurred in the presence of several other bivalent cations. Inhibiti...

Two enzymes which can remove the N-terminal pyroglutamyl residue of Thyroliberin (Glp-His-ProNHz) have been identified in brain tissue of guinea-pig and other animals. Pyroglutamate aminopeptidase I is present in cytoplasm and requires DTT and EDTA for the assay of activity. [l] Pyroglutamate aminopeptidase II has been located in synaptosomal membranes and is inhibited by EDTA [2]. A number of ...

We previously found that the myeloid cell surface glycoprotein CD13 (gp150) is identical to aminopeptidase N (EC 3.4.11.2), a widely distributed membrane-bound, zinc-dependent metalloprotease with an extracellular enzymatic domain that cleaves N-terminal amino acid residues from oligopeptides (J Clin Invest 83:1299, 1989). As a first step toward defining the function of this molecule on myeloid...

The present investigation measured the relative pressor potencies of intracerebroventricularly infused ANG II, ANG III, and the metabolically resistant analogs d-Asp(1)ANG II and d-Arg(1)ANG III in alert freely moving rats. The stability of these analogs was further facilitated by pretreatment with the specific aminopeptidase A inhibitor EC33 or the aminopeptidase N inhibitor PC18. The results ...

Regional distribution of intestinal enzymes in Nile tilapia was investigated using enzyme histochemistry. Samples from adult fish were obtained from the five major intestinal segments. Activities of maltase, leucine aminopeptidase, dipeptidyl aminopeptidase IV, lipase, non-specific esterases, and alkaline phosphatase were examined in each segment. All enzymes were present at specific sites alon...

Human coronavirus HCoV-229E uses human aminopeptidase N (hAPN) as its receptor (C. L. Yeager et al., Nature 357:420-422, 1992). To identify the receptor-binding domain of the viral spike glycoprotein (S), we expressed soluble truncated histidine-tagged S glycoproteins by using baculovirus expression vectors. Truncated S proteins purified by nickel affinity chromatography were shown to be glycos...

CD13 Ag and CD11a, CD11b, CD18 molecule expression on peripheral blood mononuclear cells (PBMC) were studied as these cells' adherent or transendothelial migration properties in three different multiple sclerosis (MS) patients groups (total 38): with clinically active MS (acute exacerbation of MS and primary chronic progressive MS (CP-MS)) and with MS remission. The control group consisted of p...

A cell surface protein expressed on melanoma cells, but not on normal melanocytes, was biochemically and functionally characterized. Microsequencing of the M(r) 143,000 affinity-purified protein revealed amino acid sequence identity to aminopeptidase N (EC 3.4.11.2). In situ expression, indirect immunofluorescence, and Western blotting demonstrated that aminopeptidase N is tightly associated wi...

Several biliary proteins have cholesterol crystallisation promoting activity. One of these glycoproteins is aminopeptidase-N, a canalicular ectoenzyme. This study attempted to localise aminopeptidase-N along the biliary tree, to assess its concentration in a series of 98 patients subjected to abdominal surgery, 40 of them without gall stones, and to correlate its concentration with cholesterol ...