To determine whether decreased alkaline phosphatase activity in the granules from neutrophils of patients with chronic myelogenous leukemia (CML) was due to an absence of enzyme or the production of defective enzyme, we compared the immunologic properties of granule alkaline phosphatase derived from patients with CML with that of normal subjects and patients with polycythemia vera (PRV). Antise...

Most histidine kinases are bifunctional enzymes having both kinase and phosphatase activities. The cytoplasmic kinase domain of EnvZ, a transmembrane histidine kinase functioning as an osmosensor in Escherichia coli, consists of two distinct functional subdomains: domain A [EnvZc(223-289)] and domain B [EnvZc(290-450)]. NMR studies demonstrated that domain A consists of a four-helix bundle serv...

Transforming growth factor β (TGFβ) causes the acquisition of epithelial-mesenchymal transition (EMT). Although the tumor suppressor gene PTEN (phosphatase and tensin homologue deleted from chromosome 10) can negatively regulate many signaling pathways activated by TGFβ, hyperactivation of these signaling pathways is observed in lung cancer cells. We recently showed that PTEN might be subject t...

We have isolated a set of complementary DNA (cDNA) clones that together encode the alkaline phosphatase of human colon cancer LS174T cells. These clones include two cDNAs isolated from a conventionally prepared oligodeoxythymidylate-primed lambda ZAP cDNA library and three cDNA clones prepared by using the polymerase chain reaction. The deduced amino acid sequence of the alkaline phosphatase pr...

conclusions based on our results, the administration of fe3o4-nps causes dose dependent adverse effects on liver. background because of their unique magnetic properties, fe3o4 nanoparticles (fe3o4-nps) have extensive applications in various biomedical aspects. investigation of the possible adverse aspects of these particles has lagged far behind their fast growing application. objectives the cu...

background recently, the clinical use of low power lasers has increased, and it is said that wound healing is accelerated by their irradiation. the aim of this study was evaluation of the effect of platelet-rich plasma on proliferation and differentiation of human dental pulp stem cells with or without ga-al-as laser. methods: in this experimental study, human lower third molar dental pulp cell...

Abstract Sepsis is defined as life-threatening organ dysfunction and damage caused by an aberrant host immune response to systemic infection. Due the abundant production of different classes inflammatory mediators, investigating role endogenous proteins that influence multiple signaling pathways might provide important clues in understanding sepsis pathogenesis. Phosphatase tensin homolog (PTEN...

RATIONALE Decreased alkaline phosphatase activity after infant cardiac surgery is associated with increased post-operative cardiovascular support requirements. In adults undergoing coronary artery bypass grafting, alkaline phosphatase infusion may reduce inflammation. Mechanisms underlying these effects have not been explored but may include decreased conversion of extracellular adenine nucleot...