Earlier in our laboratory, the role of various individual sperm impairing microorganisms on parameters and female infertility has been elucidated at higher doses. As, multiple bacterial species tend to exert more pathogenic effect comparison single organism hence, present study was carried out evaluate that if consortia these organisms can lead mice sub fertility dose. For this, impact strains ...

Changes in both external form and internal arrangement of nuclear material have been investigated in the differentiation of the sperm of the pulmonate snail, Otala lactea. Sperm head differentiation begins with a flattening of the previously spherical nucleus and a thickening of the nuclear envelope covering the anterior and posterior surfaces of that nucleus. Tail filaments can be seen in the ...

Glycoproteins were extracted from membranes of erythrocytes that displayed Tn polyagglutination and were compared chemically and immunologically with glycoproteins of group O, MN cells. Tn glycoprotein had lower than normal NANA : protein and sugar : protein ratios, as revealed by direct analysis and polyacrylamide gel electrophoresis, and displayed slower immunoelectrophoretic mobility than gl...

To increase fertilization chances compared with rivals, males are favoured to transfer accessory gland proteins to females during mating. These substances, by influencing female physiology, cause alteration of her sperm usage and remating rate. Simultaneously hermaphroditic land snails with love-darts are a case in point. During courtship, a love-dart is pierced through the partner's body wall,...

AIMS To investigate the reaction of 118 blood culture isolates of coagulase negative staphylococci with a panel of seven lectins. METHODS The interactions between the bacterial suspensions and lectins from Arachnis hypogaea (peanut agglutinin), Bauhina purpurea, Solanum tuberosum (potato starch), Triticum vulgaris (wheat germ agglutinin), Wisteria floribunda, Concanavalin ensiformis and Limul...

During murine fertilization, sperm bind to the specialized extracellular matrix of the egg, known as the zona pellucida (ZP). This matrix is composed of three major glycoproteins designated ZP1, ZP2, and ZP3. Three models for sperm-ZP binding are now under consideration. The domain-specific model posits that adhesion relies primarily on interactions between N-glycans located within the C-termin...

Human erythrocytes become agglutinable with concanavalin A (Con A) after treatment with various proteinases or neuraminidase. The extent of agglutinability achieved with different enzymes is, however, different: Pronase, papain, trypsin, neuraminidase and chymotrypsin enhance the agglutinability in decreasing order, the last being barely effective. The actions of the enzymes on band 3, the Con ...

A correlation has been observed between concanavalin A agglutination of various cell types and the presence of surface membrane galactosyltransferase (1-O-alpha-D-Galactosyl-myo-inositol:raffinose galactosyltransferase, EC 2.4.1.67) activity. Moreover, a reduction to less than 50% of cell surface galactosyltransferase activity occurred after treatment with concanavalin A; other cell surface gly...

In order to determine probiotic properties, 137 strains of lactic acid bacteria from the feces of Creole goats were screened, only six were resistant to pH 2.0 and bile salts (0.3%). Three strains identified as Lactobacillus and two as Enterococcus showed agglutination with the treated yeast. Between them, Lactobacillus DDL17, DDL19, DDL48 and Enterococcus DDE39 demonstrated high specificity in...

Lectins are cell-binding and agglutinating (glyco)proteins found in a variety of living forms. They will be defined here as homogeneous carbohydrate-binding (glyco)proteins principally of plant (seed) origin but also occurring in invertebrate (and vertebrate?) forms. In recent years, lectins have been employed in studying a wide range of biological phenomena including cell differentiation and m...