Burkholderia pseudomallei, the causative agent of melioidosis was found in a small cluster of cases in Tejuçuoca, Ceará, Brazil. Tests were carried out to determine its phenotypic characteristics: colony morphology on Ashdown agar and MacConkey agar, biochemical profile in conventional biochemical tests and API 20NE, arabinose assimilation and susceptibility testing by disk diffusion, comparing...

Methods We collected swabs from wounds in 16 ICU patients and 58 of burn ward patients during a two month period Jan.-March 2011. Clinical samples were inoculated on Blood agar and MacConkey agar. The isolates were identified with Gram stain, oxydase test and Enterosystem 18R for the gram negatives and Staphy slide test for the staphylococci. The antibiogram was performed by the disc-diffusion ...

Methods At the Infectious Disease Hospital in Constanța, between January 2014 and June 2014, urine cultures were performed in patients presenting urinary tract infections. Urine was seeded by loops calibrated technique on Columbia agar with 5% sheep blood and Drigalski agar. To identify the germs we used latex agglutination kits for Gram-positive germs and API galleries (BioMerieux) for Gram-ne...

A study of 283 isolates of gram-negative bacilli revealed a good correlation (r = -0.74) between disk diffusion zones of inhibition and agar dilution minimal inhibitory concentrations. Regression analysis suggested that strains with zone sizes =/<11 mm should be considered resistant, but 34 of 45 strains resistant by minimal inhibitory concentration (including 27 strains of Pseudomonas aerugino...

A double diffusion-in-agar test was used to investigate precipitation reactions of 75 arboviruses. Specific reactions were regularly observed with members of arbovirus groups B, California, Simbu, Turlock, Hart Park, vesicular stomatitis, and several other arboviruses as well as with a member of the Tacaribe group and a herpesvirus. The results demonstrated the feasibility of applying this tech...

An agar diffusion method using paper disks on plates seeded with Clostridium perfringens and incubated anaerobically to assay serum levels of metronidazole is described. The lowest serum metronidazole concentration that could be measured was 0.7 mug/ml, and the average error was 4% for 5 mug/ml. This assay could measure metronidazole in the presence of gentamicin or in the presence of penicilli...

Rifampin was incorporated into Middlebrook 7H10 medium either by adding an aliquot of the antibiotic into melted agar (final concentration 1.0 and 3.0 mug/ml) or by submerging a 5 or 15 mug of rifampin paper disk into 5 ml of melted agar contained in one quadrant of a Felson "X" plate. At intervals, plugs of agar were removed from the stored plates and assayed. Plates stored at 5 C for 28 days ...

background: ornithobilharziosis as one of the parasitic infections may give rise to serious economic problems in animal husbandry. the aim of the study was to prepare and compare the somatic and excretory-secretory (es) antigens of o. tur­kestanicum in gel diffusion test. methods: excretory-secretory (es) and somatic antigens of ornithobilharzia turkestanicum were prepared from collected worms ...