AIM Detection of aflatoxin B1 in Livestock compound Feed and feed ingredients by high-performance thin layer chromatography (HPTLC). MATERIALS AND METHODS Chromatography was performed on HPTLC silica gel 60 F 254, aluminum sheets by CAMAG automatic TLC sampler 4, with mobile phase condition chloroform:acetone:water (28:4:0.06). Extraction of aflatoxin B1 from samples was done as per AOAC meth...

AIMS The objective of this study was to determine if acetosyringone affected the expression of aflatoxin biosynthetic genes. METHODS AND RESULTS Two genes, nor1 and ver1, representing genes whose products are involved in early and late steps in aflatoxin biosynthesis, were examined. Two GUS (beta-glucuronidase) reporter constructs, nor1:GUS (pGAP12) and ver1:GUS (pGAP13), were used to study t...

Aflatoxin biosynthesis in the filamentous fungus Aspergillus parasiticus involves a minimum of 21 enzymes, encoded by genes located in a 70 kb gene cluster. For aflatoxin biosynthesis to be completed, the required enzymes must be transported to specialized early and late endosomes called aflatoxisomes. Of particular significance, seven aflatoxin biosynthetic enzymes are P450/monooxygenases whic...

Cottonseed is an economical source of protein and is commonly used in balancing livestock rations; however, its use is typically limited by protein level, fat content, gossypol, and the potential for aflatoxin contamination. There are numerous studies in the literature discussing gossypol and aflatoxin toxicities in livestock and processing methods for reducing gossypol levels in cottonseed. Ho...

background: the main aim of the present study was to evaluate the ameliorative effect of curcumin on aflatoxin-induced changes in caput and cauda epididymis of mice. materials and methods: aflatoxin was obtained by growing aspergillus parasiticus in sucrose - magnesium sulphate - potassium nitrate - yeast extract (smky) liquid medium. pure curcumin (97% purity) was purchased from hi-media labor...

introduction: aflatoxin b1 (afb1) is one of the most well-known hepatocarcinogens in humans. contamination of raw materials, used in the production of sausages and burgers, with aflatoxin producing molds can lead to increased level of aflatoxin in the final products and can impose hazards to human health. unfortunately, aflatoxin is resistant to heating and freezing processes, etc. and can rema...

Aflatoxins are cancer-causing, immuno-suppressive mycotoxins that frequently contaminate important staples in Zambia including maize and groundnut. Several species within Aspergillus section Flavi have been implicated as causal agents of aflatoxin contamination in Africa. However, Aspergillus populations associated with aflatoxin contamination in Zambia have not been adequately detailed. Most o...

The objective of this study was to examine the relationship between fluorescence emissions of corn kernels inoculated with Aspergillus flavus and aflatoxin contamination levels within the kernels. Aflatoxin contamination in corn has been a long-standing problem plaguing the grain industry with potentially devastating consequences to corn growers. In this study, aflatoxin-contaminated corn kerne...

Activation of ras proto-oncogenes occurs frequently in vivo in chemically induced rodent tumours, including rat hepatomas induced by aflatoxin B1. This study examines the in vitro activation of a human ras gene by this mycotoxin. A plasmid containing the human Ha-ras proto-oncogene, together with a neomycin resistance gene (pECneo), was incubated in vitro with a microsomal system generating afl...