TFIID plays a role in nucleating RNA polymerase II preinitiation complex assembly on protein-coding genes. TFIID is a multisubunit complex comprised of the TATA box binding protein (TBP) and 14 TBP-associated factors (TAFs). Another class of multiprotein transcriptional regulatory complexes having histone acetyl transferase (HAT) activity, and containing TAFs, includes TFTC, STAGA and the PCAF/...

A comparative study of several commonly-used killing, f i i g and mounting techniques was made to identify the optimum procedure for preparation of parthenogenetic adult female Caenorhabditis elegans for taxonomic examination. Nematodes were either 9 killed in hot water (95 OC) and fixed in cold fixative (22 "C) or iz) killed and fiied in hot fixative by Seinhorst's (1966) technique. Both the q...

The recruitment of TATA binding protein (TBP) to gene promoters is a critical rate-limiting step in transcriptional regulation for all three eukaryotic RNA polymerases. However, little is known regarding the dynamics of TBP in live mammalian cells. In this report, we examined the distribution and dynamic behavior of green fluorescence protein (GFP)-tagged TBP in live HeLa cells using fluorescen...

The synrift sandstone deposits of Eo-Oligocene Lemat (LMF) and Oligo-Miocene Talang Akar Formations (TAF) are the most well -known hydrocarbon reservoirs in South Sumatra Basin, Indonesia. However, only limited studies on TAF North Palembang Sub-Basin have been published. In Ridho field, is characterized by fining-up sequences inter bedded, fine to medium-grained sandstones shales overlain a co...

Abstract The Thermodynamics of Advanced Fuels – International Database (TAF-ID) was developed using the Calphad method to provide a computational tool perform thermodynamic calculations on nuclear fuel materials under normal and off-normal conditions. Different kinds fuels are considered: oxide, metallic, carbide nitride fuels. Many fission products introduced as well structural (e.g., zirconiu...

Pancreatic ductal adenocarcinoma (PDAC) is a life-threatening disease and there is an urgent need to develop improved therapeutic approaches. The role of c-Jun N-terminal kinase (JNK) in PDAC stroma is not well defined even though dense desmoplastic reactions are characteristic of PDAC histology. We aimed to explore the role of JNK in PDAC stroma in mice. We crossed Ptf1aCre/+ ;KrasG12D/+ mice ...

Transcription factor IIA (TFIIA) is a positive acting general factor that contacts the TATA-binding protein (TBP) and mediates an activator-induced conformational change in the transcription factor IID (TFIID) complex. Previously, we have found that phosphorylation of yeast TFIIA stimulates TFIIA.TBP.TATA complex formation and transcription activation in vivo. We now show that human TFIIA is ph...

Human transcription factor TFIID contains the TATA-binding protein (TBP) and several TBP-associated factors (TAF(II)s). To elucidate the structural organization and function of TFIID, we expressed and characterized the product of a cloned cDNA encoding human TAF(II)135 (hTAF(II)135). Comparative far Western blots have shown that hTAF(II)135 interacts strongly with hTAF(II)20, moderately with hT...

The mechanism of skeletal myoblast fusion is not well understood. We show that endogenous nitric oxide (NO) generation is required for myoblast fusion both in embryonic myoblasts and in satellite cells. The effect of NO is concentration and time dependent, being evident only at the onset of differentiation, and direct on the fusion process itself. The action of NO is mediated through a tightly ...

In this work, we propose a motion robust and high-speed detection pipeline which better leverages the event data. First, design an stream representation called temporal active focus (TAF), efficiently utilizes spatial-temporal asynchronous stream, constructing tensors to object motions. Then, module bifurcated folding (BFM), encodes rich information in TAF tensor at input layer of detector. Fol...